Method and device for detecting ultra trace sample based on surface-enhanced Raman spectroscopy

Abstract

The invention discloses a method and device for detecting an ultra trace sample based on surface-enhanced Raman spectroscopy (SERS). The device disclosed herein comprises an SERS optrode and a sample adaptive positioner having a groove structure with super-hyophobic characteristics. The method disclosed herein is characterized in that: a sample solution adheres to a super-hyophobic substrate without infiltration to form spherical liquid drops, but the sample solution adheres to the SERS optrode with infiltration; with the evaporation of the solvent, the sample liquid drops turns smaller and leave the super-hyophobic surface, solutes are concentrated and deposited at the head of the SERS optrode, thus the unit area concentration of the detected object is raised, and high-sensitivity analysis is realized. According to the invention, the amount (10-20 muL) of the required sample solution is few, the analysis sensitivity is high (e.g. the actual detection limit of pesticide Hostathion is only 20pg.), the method disclosed herein can be widely applied in the analysis and detection of trace materials in the fields of homeland security, environmental monitoring, food safety, medical health, etc.

Description

technical field [0001] The invention relates to the field of molecular spectrum analysis and detection, in particular to optode technology using optical fiber as a SERS carrier, super-lyophobic materials, and optode-induced evaporation pre-concentration analysis technology based on super-lyophobic materials. Background technique [0002] When light is irradiated on the material, elastic scattering and inelastic scattering occur. The scattered light of elastic scattering has the same wavelength as the excitation light, and the scattered light of inelastic scattering has components longer and shorter than the wavelength of excitation light, which is called Raman Effect; Since the vibrational spectrum is the fingerprint of the substance molecule, the Raman spectrometer made according to the Raman effect can be used to accurately and qualitatively identify samples. The analysis method of Raman spectroscopy generally does not require pretreatment of samples, and is easy to operat...

AI Technical Summary

Problems solved by technology

These shortcomings make it difficult to become a mature field analysis technology

In addition, the manufacturing technology of the SERS substrate with a higher enhancement factor also relies on micro-nano processing technology, and the cost is still high

Finally, the existing bifunctional substrates are all superhydrophobic materials, and organic solvents are often used to extract the samples to be analyzed in the field analysis

This limits the scope of application of existing technologies

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