An in-situ
chemical imaging method and
system applied to a soil drilosphere. The method comprises: S1, preparing an
earthworm burrowing box and filling same with soil; S2, selecting appropriate earthworms and placing same in the soil for culturing, such that sufficient
earthworm pores are generated in the soil; S3, sequentially providing a filter membrane (9), a DGT adsorption membrane (107) and a pH planar
optode membrane (106) on a soil profile of a drilosphere which needs to be imaged, such that the three membranes can all be fully attached to the soil profile of the drilosphere, applying
ultraviolet light under dark conditions, culturing for a period of time, and capturing a
fluorescence signal emitted by the pH planar
optode membrane (106), so as to obtain a pH
fluorescence image; S4, performing color development on the DGT adsorption membrane (107); and S5, capturing a color-developed image of the DGT adsorption membrane (107) to obtain a DGT image, and performing picture
processing on the DGT image and the pH
fluorescence image to obtain
spatial distribution images of available
phosphorus and pH in the drilosphere. The problem of difficulty in in-situ detection of a biogeochemical
cycling process driven by
earthworm activities in soil is solved.