Method for simultaneous detection of vanillin and ethyl vanillin in milk powder

A technology of ethyl vanillin and vanillin, applied in the field of simultaneous detection of vanillin and ethyl vanillin in milk powder, can solve the problems of poor solubility of ethyl vanillin, achieve high accuracy, Low matrix interference and simple pre-treatment

Inactive Publication Date: 2013-02-27
青岛谱尼测试有限公司
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Problems solved by technology

[0007] It is known from experiments that the solubility of ethyl vanillin in hot water is not good, and the solubility in methanol and ethanol is relatively high. In order to realize the simultaneous detection of the two flavors, it is urgent to find a simple, fast, high-accuracy The pretreatment method to meet the needs of the detection of two substances in milk powder

Method used

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  • Method for simultaneous detection of vanillin and ethyl vanillin in milk powder

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Experimental program
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Embodiment 1

[0025] Determination of vanillin and ethyl vanillin content in a certain brand infant formula milk powder (3 sections) of embodiment 1

[0026] 1. Analysis steps

[0027] Weigh 1.00g of milk powder sample, accurate to 0.0001g, add 10mL of absolute ethanol to a 50mL centrifuge tube, mix well, ultrasonically extract for 20min, refrigerate and centrifuge at 8000r / min for 5min, pass the supernatant through a 0.22μm microporous membrane, and wait for HPLC assay.

[0028] 2. High performance liquid chromatography conditions

[0029] Mobile phase: acetonitrile: 0.02mol / L sodium dihydrogen phosphate solution = 25:75, v / v; flow rate: 1.0mL / min; injection volume: 10μL; column temperature: 30°C; detection wavelength: 278nm.

[0030] 3. Standard working curve

[0031] Dilute the 1000μg / mL standard stock solution step by step to obtain 1.00, 2.00, 5.00, 10.0, 25.0μg / mL standard working solutions. Measured according to the above-mentioned instrument conditions, the vanillin standard cur...

Embodiment 2

[0034] Determination of vanillin and ethyl vanillin content in a certain brand infant formula milk powder (4 sections) of embodiment 2

[0035] There is ethyl vanillin in the food label ingredients of this brand infant formula milk powder (stage 4), adopt the same method as Example 1, measure the content of its vanillin and ethyl vanillin, the experimental results show that in this sample Vanillin was not detected, and the content of ethyl vanillin was 129.3mg / kg.

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Abstract

The method discloses a method for simultaneous detection of vanillin and ethyl vanillin in milk powder, including the following steps: ultrasonic extracting vanillin and ethyl vanillin in milk powder by anhydrous ethanol, precipitating protein, centrifuging the extract liquid to cross membrane, separating by a chromatographic column having a filler of octadecyl silane, detecting by a diode array detector, and quantifying by the external standard method. The method has the characteristics of simple pre-treatment, accurate qualitative determination, and fast quantification, and is suitable for simultaneous detection of vanillin and ethyl vanillin in milk powder.

Description

technical field [0001] The invention relates to a method for simultaneously detecting vanillin and ethyl vanillin in milk powder, in particular to a detection method for determining the contents of vanillin and ethyl vanillin in milk powder by high performance liquid chromatography. Background technique [0002] Vanillin (3-methoxy-4-hydroxybenzaldehyde) and ethyl vanillin (3-ethoxy-4-hydroxybenzaldehyde) are synthetic flavors, both of which are white or slightly yellow crystalline Powder, slightly soluble in water, soluble in ethanol, glycerin, ether, chloroform and spices. Vanillin has the aroma of vanilla and strong milky aroma, and the aroma of ethyl vanillin is 3-4 times that of vanillin. It is widely used in food, chocolate, ice cream, beverages and daily cosmetics to enhance flavor and stabilize Fragrance effect. According to a report by the European Commission of Experts in 2000, large doses can cause headaches, nausea, vomiting, dyspnea, and even damage to the liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 宋薇
Owner 青岛谱尼测试有限公司
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