Check patentability & draft patents in minutes with Patsnap Eureka AI!

Type detection kit and preparation method thereof for hand-foot -mouth diseases (HFMD)

A technology for detecting kits and hand, foot and mouth disease, applied to measuring devices, analysis materials, instruments, etc., to achieve the effect of simple operation

Inactive Publication Date: 2013-02-27
SHENZHEN BLOT BIOTECH
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of CGIA for the typing of hand, foot and mouth disease (HFMD) has not been reported in China.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Type detection kit and preparation method thereof for hand-foot -mouth diseases (HFMD)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 kit

[0041] Preparation of gold standard EV71VP1 and CA16VP1 antibodies: add 1% trisodium citrate 1ml to 100ml 0.01% chloroauric acid aqueous solution, heat and boil for 15-30 minutes, the solution turns from blue to red, which is colloidal gold solution, prepared to 0.01 % colloidal gold was mixed with 0.5mg / ml EV71VP1 and CA16VP1 antibodies at a ratio of 10:1, stirred electromagnetically for 30 minutes, determined the final volume, added 10% BSA to a final concentration of 1%, and continued to stir for 30 minutes. Stand at 4°C for 2 hours, centrifuge at 1500 rpm for 15 minutes at 4°C, discard the precipitate and then centrifuge at 10,000 rpm for 1 hour, discard the supernatant, and wash the precipitate with 0.01mol / LPB (1%BSA, 0.02%PEG and 0.05%NaN 3 ) was resuspended to 1 / 10 of the original volume, filtered through a filter membrane with a pore size of 0.45um, and the gold-labeled EV71VP1 and CA16VP1 antibodies were collected and sto...

Embodiment 2

[0046] Research objects: 136 HFMD patients (79 males and 57 females), aged 6 months to 8 years, were clinically diagnosed in accordance with the Ministry of Health's "Guidelines for the Prevention and Control of Hand, Foot, and Mouth Disease (2008 Edition)" in the Department of Infectious Diseases of our hospital. 30 normal controls were obtained from children who underwent physical examination in our hospital, including 17 males and 13 females, aged 6 months to 7 years, with no history of fever and diarrhea in the past two weeks.

[0047] Specimen collection and preservation: About 2 grams of stool (the size of a peanut) was collected from HFMD patients and normal control children within 3 days of clinical diagnosis, and stored in a test tube containing 3 milliliters of Hanks solution at 4°C.

[0048] Add 100 μL of blocking solution to the sample loading area of ​​the detection tool prepared in Example 1, and wait for infiltration; add 100 μL of diluted feces to be tested, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biotechnology, particularly to a type detection kit and a preparation method thereof for HFMD. According to the kit, a double-antibody sandwich principle is used, antibodies of CA16VP1 and EV71VP1 are coated in a nitrocellulose membrane and used for detecting various types of HFMD virus antigens in throat swabs or excrement of an HFMD patient. Coxsackie virus A16 type infection is diagnosed if the CA16VP1is positive, and enterovirus 71 type infection is diagnosed if the EV71VP1 is positive. The kit can be used for detecting the virus antigens in the throat swabs or the excrement of an HFMD child patient immediately and directly (within several minutes) and detecting EV71 and CA 16 pathogens simultaneously, type diagnosis for HFMD child patients can be achieved, the operation is simple, special instruments are not needed, and the kit is suitable for levels of medical institutions and disease control centers.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hand-foot-mouth disease segregation detection kit and a preparation method thereof. Background technique [0002] Hand, foot and mouth disease (HFMD) is an acute infectious disease in children with herpes on the skin of hands, feet and oral mucosa as the main symptoms. Hand, foot and mouth disease was reported abroad in 1957. In 1981, my country first reported the epidemic of hand, foot and mouth disease from Shanghai. In recent years, the prevalence of EV71 virus has been on the rise in the Asia-Pacific region. There are more than 20 types of enteroviruses that cause HFMD. Among them, Coxsackievirus A16 (Coxsckievirus A16, CA16) and enterovirus 71 (enterovirus71, EV71) are the most common. Usually, HFMD caused by EV71 infection is indistinguishable from HFMD caused by CA16 infection in terms of clinical symptoms and other aspects. Patients caused by CA16 infection can cause compl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 马伟民张永顶马新民
Owner SHENZHEN BLOT BIOTECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com