Wnt-targeted drug screening cell model, its construction and application
A cell model and drug technology, applied in the field of medicine, can solve the problems of no screening method, increased instability of the reporting system, and increased cost, so as to shorten the screening period, improve system stability, and reduce operation steps.
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[0033] The structure, construction method and application of the Wnt-targeted drug screening cell model of the present invention will be further illustrated by specific examples below. The instruments and reagents used in the experiment are as follows:
[0034] Instrument: PerkinElmer Victor3 fluorescence / chemiluminescence analyzer.
[0035] Reagents: Firefly luciferase assay kit was purchased from Promega; XAV939 and puromycin were purchased from Sigma-Aldrich; 96-well plates used for drug screening were purchased from Corning; restriction endonuclease and T4 DNA ligase were purchased From Fermentas; Sequence synthesis and sequencing were provided by Shanghai Shenggong; DMEM (high sugar) and fetal bovine serum were purchased from Hyclone (Thermo), the transfection reagent GenEscort II was purchased from Nanjing Huiji Biotech; the selected compounds were from National Small Molecules Provided by the Compound Resource Center; various antibodies were purchased from Cell Signaling Te...
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