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Ganoderma lucidum spores oil or ganoderma lucidum spore powder or quality test method of preparation thereof

A quality detection method, the technology of ganoderma lucidum spore powder, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple method, high reproducibility and strong operability

Active Publication Date: 2013-03-13
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no literature report using 1,2-dioleic acid-3-linoleic acid-triglyceride, 1-palmitic acid-2-oleic acid-3-linoleic acid-triglyceride, 1,2 -Dilinoleic acid-3-oleic acid-triglyceride, 1,3-dipalmitic acid-2-oleic acid-triglyceride content to evaluate the quality of Ganoderma lucidum spore powder or Ganoderma lucidum spore oil and its preparations

Method used

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  • Ganoderma lucidum spores oil or ganoderma lucidum spore powder or quality test method of preparation thereof
  • Ganoderma lucidum spores oil or ganoderma lucidum spore powder or quality test method of preparation thereof
  • Ganoderma lucidum spores oil or ganoderma lucidum spore powder or quality test method of preparation thereof

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Experimental program
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Embodiment 1

[0019] The research of the method for the determination of content in Ganoderma lucidum spore oil by high performance liquid chromatography of embodiment 1

[0020] (1) Instruments and reagents

[0021] Instrument: Waters2695 high performance liquid chromatography, chromatographic column: KromasilC 18 Column (4.6mm×250mm, 5μm), Sedex75 evaporative light scattering detector.

[0022] Reagents: Reference substances OOL, POL, LLO and POP were provided by Sigma-Aldrich; Ganoderma lucidum spore oil was provided by a company in Guangzhou; acetonitrile and isopropanol were chromatographically pure; other reagents were analytically pure. (2) Preparation of the test solution: Weigh 100.34mg of Ganoderma lucidum spore oil, accurately weigh (remove), use the mobile phase to constant volume to 25ml, and filter through a microporous membrane to obtain the test solution, using acetonitrile: iso A solution with a volume ratio of 53:47 of propanol is used as the mobile phase;

[0023] Prep...

Embodiment 2

[0060] Determination of OOL, POL, LLO, POP in embodiment 2 Ganoderma lucidum spore oil emulsion

[0061] Chromatographic conditions use octadecyl bonded silica gel as filler; chromatographic column: KromasilC 18 Column (4.6mm×250mm, 5μm); column temperature: 30°C; mobile phase with a solution of acetonitrile:isopropanol volume ratio of 40:60; detection by evaporative light scattering detector; flow rate 0.5ml / min; theoretical plate The number is calculated according to OOL or POL or LLO or POP peak, all greater than 2000.

[0062] Preparation of reference substance solution: Take 4.53mg, 2.85mg, 2.39mg, and 2.16mg of OOL, POL, LLO, and POP reference substances in a 10ml measuring bottle, add mobile phase solution to dissolve and dilute to the mark, and make each 1ml contain OOL, POL, LLO and POP are respectively 0.453mg / ml, 0.285mg / ml, 0.239mg / ml, 0.216mg / ml solution, namely the reference substance solution;

[0063] Preparation of the test solution: Weigh 1.15g of the oil e...

Embodiment 3

[0065] Determination of OOL content in embodiment 3 Ganoderma lucidum spore oil

[0066] Chromatographic conditions use octadecyl bonded silica gel as filler; chromatographic column: KromasilC 18 Column (4.6mm×250mm, 5μm); column temperature: 30°C; mobile phase with a solution of acetonitrile:isopropanol volume ratio of 60:40; detection by evaporative light scattering detector; flow rate 0.8ml / min; theoretical plate The number is calculated by OOL and is greater than 2000.

[0067] Preparation of reference substance solution: Take 4.87mg of OOL reference substance, place it in a 10ml measuring bottle, add mobile phase solution to dissolve and dilute to the mark, and make a solution containing 0.487mg per 1ml to obtain the reference substance solution;

[0068] Preparation of the test solution: Weigh 102.31 mg of Ganoderma lucidum spore oil, add mobile phase solution to dissolve and set the volume to 25 ml to obtain the test solution.

[0069] Determination results: Accuratel...

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Abstract

The invention discloses ganoderma lucidum spores oil or ganoderma lucidum spore powder or a quality test method of a preparation thereof. Contents of 1,2-dioleic acid-3-linoleic acid-triglyceride, 1-palmitic acid-2-oleic acid-3-linoleic acid-triglyceride, 1,2-dilinoleic acid-3-oleic acid-triglyceride and 1,3-dipalmitic acid-2-acid-triglyceride in a product are tested by a high performance liquid chromatography. 1g of ganoderma lucidum spore powder, 1g of ganoderma lucidum spore oil or the preparation equivalent to 1g of ganoderma lucidum spore powder and 1g ganoderma lucidum spore oil respectively contain 15.0mg-60.0mg of 1,2-dioleic acid-3-linoleic acid-triglyceride and 50.0mg-200.0mg of 1,2-dioleic acid-3-linoleic acid-triglyceride, or 12.0mg-50.0mg of 1-palmitic acid-2-oleic acid-3-linoleic acid-triglyceride and 40.0mg-150.0mg of 1-palmitic acid-2-oleic acid-3-linoleic acid-triglyceride or 10.0mg-50.0mg, 30.0mg-140.0mg of 1,2-dilinoleic acid-3-oleic acid-triglyceride or 5..0mg-30.0mg, 15.0mg-80.0mg of 1,3-dipalmitic acid-2-acid-triglyceride. The method can effectively test the quality of the product, and is strong in operability, high in reproducibility and strong in specificity.

Description

technical field [0001] The invention relates to a quality analysis method for medicines or health food, in particular to a quality detection method for ganoderma lucidum spore oil or ganoderma spore powder or preparations thereof. Background technique [0002] Ganoderma lucidum is the general name of Ganoderma lucidium (Leyss.ex Fr.) Karst. and Ganoderma japonicum (Fr.) Lloyd] from the family Polyproraceae (Polyproraceae) of Ganoderma. , is called top grade by "The Classic". Ganoderma lucidium spore is an extremely small spore ejected from the cap during the growth and maturity stage of Chizhi. It is the reproductive cell of Ganoderma lucidum and has all the genetically active substances of Ganoderma lucidum. Its medicinal value is also increasingly valued. The pharmacological effects of Ganoderma lucidum spores mainly include anti-tumor, immune regulation, regulation of blood lipids, and regulation and improvement of the nervous, cardiovascular and respiratory systems. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 曾荣华赖庆水陈路林蒋兆健李菁宋晓燕
Owner GUANGZHOU HANFANG PHARMA