Carboxymethyl cellulase activity determination method

A technology of carboxymethyl cellulose and sodium carboxymethyl cellulose, which is applied in the field of determination of carboxymethyl cellulose enzyme activity, can solve the problems of inaccurate determination and complicated determination process, and achieve accurate and reliable determination results, and the determination of phenomenon Clear and clear, simple and fast determination method

Active Publication Date: 2015-06-17
HUNAN XINHUI PHARMA
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AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of complex and inaccurate determination in the prior art, the present invention provides a new method for measuring the activity of carboxymethyl cellulose enzymes. By adopting the method of the present invention, the measuring process is simple and fast, and the measuring phenomenon is clear. Clear, accurate and reliable measurement results

Method used

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  • Carboxymethyl cellulase activity determination method
  • Carboxymethyl cellulase activity determination method
  • Carboxymethyl cellulase activity determination method

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Embodiment 1

[0055] We prepared Hericium erinaceus cultures as follows:

[0056] 1. Sterile culture: Hericium erinaceus cultures are sterilized by medium, inoculated in a 100,000-class clean area, and cultured under sterile conditions for 45 days at a temperature of 25°C to prevent pollution;

[0057] 2. Secondary cultivation: after the primary cultivation, the culture of Hericium erinaceus is obtained, and then transferred to a plastic bag and cultivated in the cultivation room for 7 days at a cultivation temperature of 25°C, then taken out;

[0058] 3. Low-temperature drying: under the condition of 45℃, adopt vacuum low-temperature continuous drying technology to dry;

[0059] 4. Low-temperature sterilization: Sterilize at a temperature of 45°C, and use ultraviolet sterilization to sterilize.

[0060] Preparation of reference substance solution: Take an appropriate amount of anhydrous glucose reference substance dried at 105°C to constant weight, weigh it accurately, add water to make a...

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Abstract

The present invention relates to a carboxymethyl cellulase activity determination method. According to the present invention, heriacium culture active ingredient acidic carboxymethyl cellulase activity determination influence factors such as a chromogenic agent use amount, a chromogenic time, a standing time, a carboxymethylcellulose sodium substrate concentration, an enzymatic reaction temperature, an enzymatic reaction time, an enzyme solution dilution degree and the like, and precision and adaptability of the method are inspected so as to finally determine an acidic carboxymethyl cellulase activity determination method. The determination method has characteristics of simpleness, rapidness, clear determination phenomenon, and accurate and reliable determination result.

Description

technical field [0001] The invention relates to a method for measuring cellulase activity, in particular to a method for measuring carboxymethyl cellulase activity (CMCNa-DNS). Background technique [0002] Cellulase (Cellulase) is a multi-component compound enzyme, and the composition and proportion of each component of cellulase from different sources are quite different. Generally speaking, cellulase comprises exo-beta-1,4-glucanase (Exop-1,4-glucanase, E C3.2.1.91), endo-beta-1,4-glucanase ( Endop-1, 4-glucanase, E C3.2.1.4) and cellobiase (Cellobiase, E C3.2.1.21). The proportions of the three enzyme components in cellulase products from different sources are different, so the final apparent enzyme activity will be different. At the same time, the substrates of cellulase are also relatively complex, resulting in many methods for measuring cellulase activity, and the methods are complicated and not uniform. Commonly used methods are: carboxymethyl cellulose saccharifi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 何述金常耀台贾林周代俊周准
Owner HUNAN XINHUI PHARMA
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