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Construction method of HPLC fingerprint of caesalpinia sappan medicine material and preparation thereof

A technology of fingerprint spectrum and construction method, applied in the field of drug analysis, can solve problems such as lack of unified standards, complex components, and inability to truly reflect the quality of medicinal materials.

Inactive Publication Date: 2013-03-20
山西省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For Sappania medicinal materials, due to the complexity of its components, a specific component cannot truly reflect the quality of the medicinal materials, and there is no unified standard for comprehensively studying its fingerprints to control quality

Method used

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  • Construction method of HPLC fingerprint of caesalpinia sappan medicine material and preparation thereof
  • Construction method of HPLC fingerprint of caesalpinia sappan medicine material and preparation thereof
  • Construction method of HPLC fingerprint of caesalpinia sappan medicine material and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] 1.1 Instruments and reagents

[0070] High performance liquid chromatography, water is ultrapure water. The reference substance Brazilian hematoxylin was purchased from China National Institutes for Food and Drug Control, Brazilian hematoxylin (batch number 111881-201001, purity 90.4%).

[0071] 1.2 Preparation of the test solution

[0072] Accurately weigh 15 mg hematoxylin powder injection, dilute with water to 100 ml, shake well, and obtain the test solution for powder injection.

[0073] 1.3 Preparation of reference solution:

[0074] Accurately weigh brazilian hematoxylin, add methanol to dissolve, obtain reference substance solution, the content of brazilian hematoxylin in this solution is 40 μ g / ml.

[0075] 1.4 Fingerprint analysis

[0076] Chromatographic conditions: Hypersil C18 column, 4.6mm×250mm, 5μm; detection wavelength 285nm, mobile phase flow rate 1.0mL min -1 , the column temperature is room temperature; the mobile phase A is methanol, the mobile ...

Embodiment 2

[0081] 1.1 Instruments and reagents

[0082] High performance liquid chromatography, water is ultrapure water. The reference substance protohematoxylin B was purchased from China National Institutes for Food and Drug Control, protohematoxylin B (batch number 111882-201001, purity 84.1%).

[0083] 1.2 Preparation of the test solution

[0084] Prepare hematoxylin medicinal material water extract, obtain extract solution for testing;

[0085] 1.3 Preparation of reference solution:

[0086] Protohematoxylin B was accurately weighed, dissolved in methanol to obtain a reference solution, and the content of protohematoxylin B in the solution was 40 μg / ml.

[0087] 1.4 Fingerprint Analysis

[0088] 1.4.1 Fingerprint analysis of the test solution

[0089] Chromatographic conditions: Hypersil C18 column, 4.6mm×250mm, 5μm; detection wavelength 250nm, mobile phase flow rate 1.0mL min -1 , the column temperature is room temperature; the mobile phase A is methanol, the mobile phase B ...

Embodiment 3

[0093] 1.1 Instruments and reagents

[0094] High performance liquid chromatography, water is ultrapure water. The reference substance protohematoxylin B was purchased from China National Institutes for Food and Drug Control, protohematoxylin B (batch number 111882-201001, purity 84.1%).

[0095] 1.2 Preparation of the test solution

[0096] Prepare the ethanol extract of Sappania medicinal material, and obtain the test solution of the extract;

[0097] 1.3 Preparation of reference solution:

[0098] Protohematoxylin B was accurately weighed, dissolved in methanol to obtain a reference solution, and the content of protohematoxylin B in the solution was 40 μg / ml.

[0099] 1.4 Fingerprint Analysis

[0100] 1.4.1 Fingerprint analysis of the test solution

[0101] Chromatographic conditions: Hypersil C18 column, 4.6mm×250mm, 5μm; detection wavelength 260nm, mobile phase flow rate 1.0mL min -1 , the column temperature is room temperature; the mobile phase A is methanol, the m...

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Abstract

The invention relates to a construction method of a traditional Chinese medicine fingerprint, and specifically relates to construction method of a high performance liquid chromatography (HPLC) fingerprint of a caesalpinia sappan medicine material and a preparation thereof. The invention belongs to the technical field of medicine analysis. The construction method comprises the steps that: (1) a sample solution is prepared; (2) a control solution is prepared; (3) a sample fingerprint is constructed; and (4) a control fingerprint is constructed. According to the method provided by the invention, Brazil hematoxylin or original hematoxylin B is adopted as a quantitative quality control indicator or a reference peak. With the constructed HPLC fingerprint, relative qualities of the cast majority of substances in the caesalpinia sappan medicine material and the preparation thereof can be effectively characterized. The HPLC fingerprint construction method provided by the invention has important significance upon novel medicine research and development of anti-tumor medicines.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of traditional Chinese medicines, in particular to a method for building high-performance liquid chromatography (HPLC) fingerprints of sumac medicinal materials and preparations thereof, and belongs to the technical field of drug analysis. Background technique [0002] Sumac (Caesalpinia sappan L.) is the dry heartwood of leguminous plants, and is an ancient natural medicinal material commonly used in Asian countries. It is mainly used clinically to treat cardiovascular diseases, such as senile stroke, antithrombotic pain relief, bruises, irregular menstrual blood, anti-inflammatory, etc., and is used as a red dye. [0003] The fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum of a certain type or several types of components that are common in a certain Chinese medicinal material and its preparations. At the present stage, most of the active ingredients of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88
Inventor 徐建国阎克里朱秀卿史艳春张少云乔丽娟郭素堂田艳伟李轲徐笑寒
Owner 山西省肿瘤医院