Fagus pashanica tissue culture rapid propagation method

Abstract

The invention discloses a Fagus pashanica tissue culture rapid propagation method which comprises the following steps: picking Fagus pashanica branch buds as explants; peeling off all scales of the buds so as to expose the bud points; disinfecting for 5min in 0.1% of mercury bichloride; and inoculating in a culture medium with WPM+NAA (Naphthalene Acetic Acid) of 1.0mg / L, 6-BA of 2.0mg / L and carbon powder of 2.0mg / L. After the buds are inoculated for 15 days, leaves grow up, and after 45 days, the leaves expand and grow larger and the leaves are light green in color and grow exuberantly; after being continuously cultivated for 60 days, white root systems grow out from the roots, the hair roots are obvious, the root systems are developed, and complete plants are grown and can be acclimatized and transplanted. The method has the following benefits that firstly, a research material is single in resource and is accordant in clone genetic background; secondly, extremely fewer plant materials are used, so that the materials are easy to acquire with economy, convenience and high efficiency; thirdly, the cultivation condition is controllable, and anniversary test or production can be carried out; and fourthly, the Fagus pashanica grows rapidly with short cycle and strong repeatability; and fifthly, the management is convenient, and the automation control is facilitated.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Bashanshui Qinggang. Background technique [0002] Water green, also known as beech, is a general name for plants of the genus Fagus L. of the angiosperm family Fagaceae. Water green is a widely distributed tree species worldwide: F.engleriana, F.orientalis and F.sylvatica connect Europe Subcontinent; F. grandifolia and Nothofagus across the northern and southern hemispheres [46] . Shuiqinggang is a tree or shrub; the buds have scales, long and pointed; the leaves fall off, alternate and serrated; the flowers open before the leaves; the male flowers are arranged in a stalked and pendulous capitulum; the perianth is bell-shaped, 5-7 lobed ; stamens 8-16; female flowers usually in pairs, born in axillary, stalked involucre; perianth 5-6-lobed and connate with ovary; ovary inferior, 3-loculed, 3-style, fruit with 2 nuts, Enclosed in a woody, prickly or warty involucre, neatly...

AI Technical Summary

Problems solved by technology

[0004] The fruit size of this species is obvious, it is difficult to collect, and the natural germination rate is lower than 3%.