Technique for preparing Sudan grass protoplast by using vacuum enzymolysis method

A technology of protoplast and enzymatic hydrolysis, which is applied in the direction of plant cells, etc., to achieve the effect of good cell integrity, less cell death, and simple method

Inactive Publication Date: 2013-04-03
ANHUI SCI & TECH UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of Sudan grassland biomass has not been reported yet, the establishment of the preparati

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1: Preparation of Sudangrass S722 Protoplast

[0018] 1. Preparation of sudangrass seedlings: select plump sudangrass S722 seeds, plant them in a nutrient bowl, and cultivate for 8 days at 25°C.

[0019] 2. Treatment of young leaves and stems: Cut 1g of young leaves and stems of Sudangrass S722, and cut the leaves into about 1mm length with a blade.

[0020] 3. Vacuum enzymatic hydrolysis: Put the cut stems and leaves into a triangular flask with 10mL enzymatic hydrolysis solution in a vacuum environment (-0.1MPa) for 1 hour. After taking it out, cover the mouth of the triangular flask with foil and put it into 28 ℃ shaker, rotate speed 40rpm, enzymatic hydrolysis for 3 hours. (Enzymatic hydrolysis solution formula: 0.645M mannitol, 10mM potassium chloride, 1mM calcium chloride, 8mM 2-morpholineethanesulfonic acid, pH5.5, 1.5% cellulase and 0.3% isolating enzyme R-10).

[0021] 4. Protoplast dissociation: Pour out the enzymolysis solution, add 10ml dissociation solutio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a technique for preparing a Sudan grass protoplast by using a vacuum enzymolysis method, belonging to the field of biotechnology. The technique comprises the steps of: chopping spires and stems of the Sudan grass in a certain size after the Sudan grass seeds sprout; and performing enzymolysis on cell walls of both the spires and the stems by utilizing the vacuum enzymolysis method so as to separate out a plenty of Sudan grass protoplasts. By utilizing the method, a plenty of Sudan grass protoplasts with complete cells can be prepared conveniently and rapidly.

Description

technical field [0001] A vacuum enzymolysis method for preparing protoplasts of Sudan grass belongs to the field of biotechnology, more specifically, it is a technology for preparing a large amount of protoplasts by using cell wall lyase to crack the cell walls of young leaves and stems of Sudan grass in a vacuum environment. Background technique [0002] Protoplast refers to a cell from which the cell wall has been removed or a naked cell surrounded by a plasma membrane. Mass preparation of protoplasts is an important basis for cell tissue culture and plant gene function research. The preparation method of Sudan grassland protoplasts has not been reported yet, and the establishment of the preparation method of Sudan grassland protoplasts is conducive to the further development of related research on Sudan grass. [0003] Contents of the invention [0004] The purpose of the present invention is to adopt a method of vacuum enzymolysis to prepare a large amount of protopl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/04
Inventor 李杰勤王丽华詹秋文周青万海波王茂辉
Owner ANHUI SCI & TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap