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Anabaena flos-aquae embedding immobilization method

A technology of blooming Anabaena, conical flask, applied in microorganism-based methods, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the weakening of activity, mass transfer limitations, affecting algal morphology and distribution and other issues, to achieve good results, the effect of nitrogen and phosphorus removal

Inactive Publication Date: 2013-04-03
SICHUAN AGRI UNIV
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Problems solved by technology

[0004] Immobilization will also have some impact on algae. Immobilized algal cells usually grow slower than free cells, but their growth cycle is longer and the final yield is higher than free cells; immobilization may cause mass transfer limitations, resulting in a decrease in growth rate ; Immobilization improves the synthesis of algae, and can maintain the activity of algae cells for a long time; reduces the catabolism of algae, because after immobilization, the cell activity is restricted, the activity is weakened, the energy consumption is reduced, and the catabolism is reduced; The shape and distribution of algae, the immobilized carrier has different effects on the shape of different algae species, but this effect is almost small, and the distribution of algae in the carrier is uneven

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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with specific embodiments.

[0026] 2.1 Cultivation of Anabaena blooms

[0027] BG11 medium is used to cultivate Anabaena algae, and its formula is as follows:

[0028] Table 1 BG11 medium formula

[0029]

[0030] Table 2 A5+Co solution formula

[0031]

[0032] According to the characteristics that Anabaena blooms have heterocysts and are affected by nitrogen in the growth environment, Anabaena blooms were introduced into nitrogen-enriched medium and nitrogen-deficient culture concentration respectively, and the growth of Anabaena blooms in a nitrogen-deficient environment was observed. Growth and heterocyst changes.

[0033] figure 1 It is the result of observing the morphology of Anabaena blooms under nitrogen-enriched and nitrogen-deficient conditions under the oil microscope. It can be clearly observed that there are basically no heterocysts in Anabaena blooms in the nitrogen-rich me...

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Abstract

The invention discloses an Anabaena flos-aquae embedding immobilization method which comprises the following steps: (1) pretreating Anabaena flos-aquae; (2) performing adsorption immobilization; (3) performing embedding immobilization; and (4) stabilizing. The embedding method disclosed by the patent of the invention is adapted to the physiological characteristics of Anabaena flos-aquae, so that the favorable physiological activity of Anabaena flos-aquae can be kept; the optimal embedding ratio provided by the invention ensures that the size, expandability, hydrophilicity, mass transfer efficiency and nitrogen and phosphorus removal efficiency of granules can be kept at satisfactory levels; and the embedding granules have favorable nitrogen and phosphorus removal effect.

Description

technical field [0001] The invention belongs to the technical field of algae immobilization, and relates to an embedding and immobilization method of Anabaena blooms, and the embedding particles have good nitrogen and phosphorus removal effects. Background technique [0002] Algae immobilization technology is developed from immobilized cell technology. It uses physical or chemical means to locate free algae cells in a limited space area, making it a method that not only maintains its own metabolic activity, but also can be processed after continuous reaction. Biological systems for recycling and reuse. A series of biological characteristics of algae determine its important position in sewage purification and resource utilization, and immobilized algae technology is an important means to realize the development of algae and apply it to various fields. In terms of sewage treatment, immobilized algae will be widely used in the denitrification and phosphorus removal of sewage, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/08C12R1/89
Inventor 王应军邹晓波李彬邓仕槐伍均张延宗肖鸿李远伟张小洪沈飞
Owner SICHUAN AGRI UNIV
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