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Novel method for purifying wild codonopsis pilosula saponin

A technology of ginsenosides and a new method, applied in the field of natural organic chemistry, can solve the problems of unstable product quality and low purity

Inactive Publication Date: 2013-04-17
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In China, solvent extraction or microwave extraction is generally used to purify Codonopsis saponins with macroporous resins, which have the disadvantages of low purity and unstable product quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: A kind of new method adopting biological enzymolysis, ionic liquid extraction, ultra-high pressure purification Codonopsis saponin, its steps are as follows:

[0016] (1) 50g Codonopsis root is pulverized to 20 mesh, using compound cellulase, the enzyme dosage is 0.5% of the substrate, and heat preservation and precipitation for 2h;

[0017] (2) Extract with ionic liquid 1-butyl-3-methylimidazolium salt at 70°C for 1 hour to obtain a solid-liquid mixture;

[0018] (3) Pour the mixture into an ultra-high pressure container, and process it for 2 minutes under the condition of ultra-high pressure 200MPA;

[0019] (4) Vacuum-dry the obtained liquid at 60°C to obtain crude saponin;

[0020] (5) Dissolve the obtained crude product powder in methanol and add an appropriate amount of silica gel to stir, dry and pass through a 60-mesh sieve, dry-load the sample, and perform chromatography on an industrial-grade column with a 200-mesh silica gel column;

[0021] (...

Embodiment 2

[0023] Embodiment 2: A kind of new method that adopts biological enzymolysis, ionic liquid extraction, ultra-high pressure to purify Codonopsis saponin, its steps are as follows:

[0024] (1) 50g Codonopsis root is crushed to 30 mesh, using compound cellulase, the enzyme dosage is 0.8% of the substrate, heat preservation and precipitation for 3h;

[0025] (2) Extract with ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate at 80°C for 1.5 hours to obtain a solid-liquid mixture;

[0026] (3) Pour the mixture into an ultra-high pressure container, and process it for 3 minutes under the ultra-high pressure 300MPA condition;

[0027] (4) Vacuum-dry the obtained liquid at 60°C to obtain crude saponin;

[0028] (5) Dissolve the obtained crude product powder in methanol and add an appropriate amount of silica gel to stir, dry and pass through an 80-mesh sieve, apply the sample in a dry method, and perform chromatography on an industrial-grade column with a 300-mesh silica gel...

Embodiment 3

[0031] Embodiment 3: A kind of new method adopting biological enzymolysis, ionic liquid extraction, superhigh pressure to purify Codonopsis saponin, its steps are as follows:

[0032] (1) 100g Codonopsis root is crushed to 40 mesh, using compound cellulase, the enzyme dosage is 1% of the substrate, heat preservation and precipitation for 4h;

[0033] (2) Extract with ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate at 85°C for 2 hours to obtain a solid-liquid mixture;

[0034] (3) Pour the mixture into an ultra-high pressure container, and process it for 4 minutes under the condition of ultra-high pressure 400MPA;

[0035] (4) Vacuum-dry the obtained liquid at 60°C to obtain crude saponin;

[0036] (5) Dissolve the obtained crude product powder in methanol and add an appropriate amount of silica gel to stir, dry and pass through a 60-mesh sieve, dry-load the sample, and perform chromatography on an industrial-grade column with a 200-mesh silica gel column;

[003...

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PUM

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting codonopsis pilosula saponin from wild codonopsis pilosula in great khingan, in particular to a novel method for extracting codonopsis pilosula saponin through biological enzymolysis, ionic liquid extraction and ultrahigh pressure purification. The method has the advantages that 1, a biological enzymolysis technology is adopted to allow that effective components in cells are dissolved out fully; 2, the ionic liquid extraction is adopted, and ionic liquid has a good effective component dissolving ability, and has double functions of a solvent and a catalyst; and 3, the ultrahigh pressure extraction is conducted at a room temperature, so that structural changes, losses and physiological activity reduction of the effective components caused by a heating effect are avoided; and dissolving-out of impurity components is reduced, and the yield of saponin is increased by the ultrahigh pressure technology. The method adopts the biological enzymolysis, ionic liquid extraction and ultrahigh pressure coupling technology, the biological enzymolysis is conducted for dissolving out saponin fully, then the ionic liquid catalytic extraction is conducted, the ultrahigh pressure purification is conducted, and finally secondary purification is conducted by silica gel column chromatography, so that the purity of codonopsis pilosula saponin is increased to 99.5%.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting Codonopsis saponins from wild Codonopsis pilosula in Daxing'an Mountains, in particular to a new method for purifying Codonopsis saponins by utilizing biological enzymolysis, ionic liquid extraction and ultra-high pressure. Background technique: [0002] Codonopsis pilosula, a herbaceous twining vine with white latex. The root is carrot-shaped and round, about 30 cm long, often branched in the middle. The stem is about 1.5 meters long, with many branches and glabrous. Alternate leaves, ovate or narrowly ovate, 1.2-6.5 cm long, 0.6-5 cm wide, with wavy serrations on the edges, dense or sparse pubescent hairs on both sides; petiole 0.6-2.5 cm long, often sparsely spreading Short hairs, 1-3 flowers on the top of branches, calyx glabrous, 5 lobes, narrowly oblong or oblong-lanceolate, 1.6-1.8 cm long, corolla light yellow-green, broadly campanulate, 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/344A61K125/00
Inventor 张亚红姚德坤姚德利万莉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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