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Core-shell nanogold biological probe with high SERS (surface enhanced Raman scattering) effect and preparation and application thereof

A technology of nano-biological probes and nano-gold probes, which is applied in the field of preparation of core-shell nano-gold bioprobes, can solve problems that limit the development and application of SERS technology, and achieve good repeatability

Inactive Publication Date: 2013-04-17
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, lithography technology, self-assembly technology, orderly assembly technology, etc. are used to prepare active substrates, but all have their own shortcomings, which limit the development and application of SERS technology.

Method used

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  • Core-shell nanogold biological probe with high SERS (surface enhanced Raman scattering) effect and preparation and application thereof
  • Core-shell nanogold biological probe with high SERS (surface enhanced Raman scattering) effect and preparation and application thereof
  • Core-shell nanogold biological probe with high SERS (surface enhanced Raman scattering) effect and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Add 4 μL, 100 μM SH-polyA DNA to 100 μL, 10 nM gold nanoparticles with a particle size of 15 nm, shake slightly at room temperature overnight; then add 10 μL of aging solution 0.1M PB (pH7.4) solution, shake at 350 rpm / min at room temperature for 30 min, then add Add 20 μL of 2M NaCl in four times with an interval of 30 minutes, and shake overnight at room temperature at 350 rpm / min; centrifuge and wash three times at 4°C, 12,000 rpm / min, for 20 minutes. Add 0.1M PHTH small molecule solution to 500 μL of the above solution, shake at 350 rpm / min at room temperature, and adsorb for 2-3 days. Take 100 μL of the above solution and add 50 μL of 1% PVP, 25 μL of 10 mM NH 2 OH·HCl, 25 μL 5 mM HAuCl 4 , mixed and shaken for 1 min, 20 ° C, 4000 rpm / min, centrifuged and washed three times for 6 min each time, the washing liquid was MilliQ water, and then resuspended in 100 μL MilliQ water; A core-shell nano-gold Raman probe with highly enhanced characteristic peaks of pyroxine ...

Embodiment 2

[0039]Add 4 μL, 100 μM SH-polyA DNA to 100 μL, 10 nM gold nanoparticles with a particle size of 15 nm, shake slightly at room temperature overnight; then add 10 μL of aging solution 0.1M PB (pH7.4) solution, shake at 350 rpm / min at room temperature for 30 min, then add Add 20 μL of 2M NaCl in four times with an interval of 30 minutes, and shake overnight at room temperature at 350 rpm / min; centrifuge and wash three times at 4°C, 12,000 rpm / min, for 20 minutes. Add 0.1M phthalazine PHTH small molecule solution to 500 μL of the above solution, shake at 350 rpm / min at room temperature, and absorb for 2-3 days. Take 100 μL of the above solution and add 50 μL of 1% PVP, 25 μL of 10 mM NH 2 OH·HCl, 25 μL 5 mM HAuCl 4 , mixed and shaken for 1 minute, 20°C, 4000rpm / min, centrifuged and washed three times for 6 minutes each time, the washing liquid was MilliQ water, and then resuspended in 100μL MilliQ water; A core-shell nano-gold Raman probe with highly enhanced characteristic peak...

Embodiment 3

[0041] Add 4 μL, 100 μM SH-polyA DNA to 100 μL, 10 nM gold nanoparticles with a particle size of 15 nm, shake slightly at room temperature overnight; then add 10 μL of aging solution 0.1M PB (pH7.4) solution, shake at 350 rpm / min at room temperature for 30 min, then add Add 20 μL of 2M NaCl in four times with an interval of 30 minutes, and shake overnight at room temperature at 350 rpm / min; centrifuge and wash three times at 4°C, 12,000 rpm / min, for 20 minutes. Add 0.1M DTNB small molecule solution to 500 μL of the above solution, shake at 350 rpm / min at room temperature, and adsorb for 2-3 days. Take 100 μL of the above solution and add 50 μL of 1% PVP, 25 μL of 10 mM NH 2 OH·HCl, 25 μL 5 mM HAuCl 4 , mixed and shaken for 1 minute, 20°C, 4000rpm / min, centrifuged and washed three times for 6 minutes each time, the washing liquid was Milli-Q water, and then resuspended in 100μL Milli-Q water; the particle size prepared by this method was 40-50nm, and the concentration was 1nM...

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Abstract

The invention discloses a core-shell nanogold biological probe with a high SERS (surface enhanced Raman scattering) effect and a preparation and an application thereof. The preparation comprises the following steps: assembling a section of DNA sequence and Raman micromolecules on the surface of nanogold with a small particle diameter; and further generating a gold shell with a certain thickness on the surface of a core of the nanogold, wherein a gap with a certain size exists between the core and the shell, the Raman micromolecules are in the gap, and due to specificity of the structure, high-efficiency and uniform SERS signals are obtained. The Raman micromolecules are in the gap with the fixed size between the core and the shell of the nanogold, so that the SERS signals generated in areas of the molecules, namely hotspot areas, are basically consistent and have good repeatability; the biological molecules are assembled on the surface of the prepared core-shell nanogold biological probe, so that a surface receptor of a target cell can be recognized specifically, the target cell can be detected and imaged by a laser Raman spectroscopy, and Raman scattering value increase on the obtained surface can show the expression of the surface receptor of the target cell efficiently; and the core-shell nanogold biological probe is also applicable to biological sensor detection, biomolecular detection and other research fields.

Description

technical field [0001] The invention belongs to the field of functionalization and application of nanometer materials, and relates to a preparation method of a core-shell nano-gold biological probe with high SERS effect, which can be applied to the fields of biomolecular detection, cell imaging and the like. Background technique [0002] Surface Enhanced Raman scattering (SERS) refers to the phenomenon that the Raman signal of small molecules on the rough metal surface is enhanced. This phenomenon has been widely used in the fields of surface science, analytical science, and biological science to provide molecular-level information for in-depth characterization of the structures and processes of various surfaces (interfaces), such as identifying the bonding of molecules or ions on the surface , configuration and orientation, and the surface structure of the material. Regarding the enhancement mechanism of SERS, although there is still controversy so far, the electromagnetic...

Claims

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Application Information

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IPC IPC(8): G01N21/65B82Y40/00
Inventor 颜娟宋世平樊春海何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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