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Crop variety identity card management method

A management method and crop technology, applied in the field of molecular identification of crop varieties and variety quality management, can solve problems such as difficulty in accurately distinguishing, inability to achieve uniqueness requirements, and staying.

Active Publication Date: 2015-05-06
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, most of the construction of ID cards of crop varieties has been based on the construction of DNA fingerprints by molecular means. Although this ID card at the DNA molecular level can reflect the essential differences of varieties to a certain extent, it cannot Due to the absence of important commodity information, there are great limitations in application; in addition, it is difficult to accurately distinguish some varieties with close kinship, such as near isogenic lines, that is, the uniqueness requirement cannot be achieved; thirdly, due to The research methods of various scientific research institutions are different, resulting in the establishment of different varieties of ID card models, lack of versatility, unable to meet the requirements of versatility and traceability

Method used

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  • Crop variety identity card management method
  • Crop variety identity card management method
  • Crop variety identity card management method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0071] Example 1: ID number of rice variety Wandao 153 (National Approved Rice 2008013):

[0072] OS11002008172424146634457711442517, which means: Wandao 153 is rice (OS), subspecies is indica (1), variety category is hybrid (1), variety approval area is China (00), variety approval time is 2008 ( 2008), the DNA fingerprint of the variety is 172424146634457711442517, which is a non-transgenic variety.

example 2

[0073] Example 2: The ID number of the transgenic insect-resistant cotton variety Ezamian No. 10 (National Approved Cotton 2005014):

[0074] GS1100200524324113233331433131132534T-Bt, which means: Ezamian No. 10 is cotton (GS), the cultivated species is upland cotton (1), the variety category is conventional species (1), the initial approval area of ​​the variety is China (00), the variety The initial approval time was 2005 (2005), and the DNA fingerprint of the variety was 24324113233331433131132534, which is a transgenic Bt gene insect-resistant variety (T-Bt).

[0075] In addition to expressing the ID card number in a string of numbers (or letters), it can also be expressed in the form of a barcode, two-dimensional code or RIFD electronic chip. According to the relevant standards of China's article coding, the barcode is encoded with 128 codes. For example, the ID number of Wandao 153 is OS11342005123456789A87B54321123456, and the ID number of Ezamian No. 10 is GS1100200524...

Embodiment 1

[0077] Example 1, ID card construction of hybrid rice Wandao 153 (National Approved Rice 2008013):

[0078] 1. Rice genomic DNA:

[0079] Take a few rice seeds and germinate at 30°C. One week later, transfer a single seedling into a 2.0 mL centrifuge tube; add liquid nitrogen and use a glass rod to grind until powdery, add 700 μL 65°C preheated DNA extraction to the centrifuge tube solution, incubate at 65°C for 1 h, mix by inverting every 15 min; add an equal volume of chloroform:isoamyl alcohol (24:1) mixture, mix gently, and let stand at room temperature for 15 min; centrifuge at 10,000 g for 15 min, Pipette the supernatant into another 1.5 mL centrifuge tube, add an equal volume of isopropanol to mix, place at -20°C for 30 min, centrifuge at 10,000 g at 4°C for 10 min, discard the supernatant, and add 200 μL of 70% Wash the precipitate with ethanol; centrifuge at 10,000 g for 10 min, discard the ethanol, dry at room temperature, add 50 μL of sterilized water, fully dissol...

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Abstract

The invention provides a crop variety identity card management method, which is characterized by comprising the following steps of: collecting and storing crop variety identity information into a server; converting the crop variety identity information stored in the server into a readable digital code, a bar code or a two-dimensional code which correspond one by one by a data converter; identifying the collected crop variety by the corresponding digital code, bar code or two-dimensional code in a circulation link; then, reading the digital code, the bar code or the two-dimensional code of the crop variety by an information reading terminal; and displaying the identity information of the crop variety in a written information mode by the data converter, wherein the written information mode is collected information which is stored in the server and is not converted. According to the crop variety identity card management method disclosed by the invention, the crop variety identity information is constructed and is identified in the forms, such as the two-dimensional code, the two-dimensional code is identified by the information reading terminal, the crop variety identity information is read, and convenience is provided for the scientific and standard management of the crop variety.

Description

technical field [0001] The invention belongs to the technical field of crop variety molecular identification and variety quality management. Specifically, it uses the basic commodity information of the crop variety, combines the variety DNA fingerprint information and the specific gene identification information, and constructs an ID card of the crop variety and encodes it; it is a crop variety ID card. Provide technical support for breeding, experimentation, quality and market management, and property rights protection. Background technique [0002] Variety seeds are important means of agricultural production. Different varieties have different production capacity and adaptability. One seed can change the world, which is the importance of good crop variety seeds. "Crop variety" refers to a plant population that has been artificially selected or discovered and improved, has consistent morphological characteristics and biological characteristics, and relatively stable genetic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06K7/10G06K19/06
Inventor 杨剑波陆徐忠马琳倪金龙马卉倪大虎李莉张小娟宋丰顺汪秀峰杨亚春
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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