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Preparation method for polysaccharide microspheres based on medicine cross-linking

A technology of polysaccharides and microspheres, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, etc. Toxicity and other issues, to achieve the effect of short processing cycle, protection of biological activity, and guaranteed use safety

Inactive Publication Date: 2013-04-24
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing chemical cross-linking agents have different degrees of chemical toxicity. On the one hand, the cross-linking process will change the molecular structure of the material and denature the material; on the other hand, it will easily lead to the denaturation and inactivation of the embedded cell growth factors.
In addition, the cross-linking agent that does not participate in the function is not easy to be removed, which will cause residue and increase the toxicity of the material

Method used

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  • Preparation method for polysaccharide microspheres based on medicine cross-linking
  • Preparation method for polysaccharide microspheres based on medicine cross-linking
  • Preparation method for polysaccharide microspheres based on medicine cross-linking

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Prepare 100 ml of hyaluronic acid aqueous solution with a mass / volume concentration of 0.5% at room temperature, add 0.05 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, stir well, 37 o Activation at C for 0.5 hours;

[0033] (2) Prepare 10 ml of gyrase solution with a mass / volume concentration of 0.5%, and add it dropwise to the activated hyaluronic acid solution, 37 o Stir the reaction at C for 2 hours, lyophilize after dialysis for 3 days;

[0034] (3) Dissolve the lyophilized hyaluronic acid in step 2 in 2 ml of water with a mass / volume concentration of 1%, add it to 50 ml of paraffin oil, add 1 ml of Span 80, and perform ultrasonic emulsification for 2 minutes;

[0035] (4) Add 0.5 ml of coumarin aqueous solution with a mass / volume concentration of 1% to the emulsion obtained in step 3, and continue ultrasonic emulsification for 2 minutes;

[0036] (5) Mix the emulsion obtained in step 4 at 40 o C was stirred and volatilized at 800 rpm overnight, and the...

Embodiment 2

[0038] (1) Prepare 100 ml of hyaluronic acid aqueous solution with a mass / volume concentration of 1% at room temperature, add 0.25 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, stir well, 37 o Activation at C for 1 hour;

[0039] (2) Prepare 10 ml of gyrase solution with a mass / volume concentration of 0.5%, and add it dropwise to the activated hyaluronic acid solution, 37 o Stir the reaction at C for 2 hours, lyophilize after dialysis for 3 days;

[0040] (3) Dissolve the lyophilized hyaluronic acid in step 2 in 2 ml of water with a mass / volume concentration of 1%, add it to 50 ml of paraffin oil, add 1 ml of Span 80, and perform ultrasonic emulsification for 5 minutes;

[0041] (4) Add 0.5 ml of coumarin aqueous solution with a mass / volume concentration of 2% to the emulsion obtained in step 3, and continue ultrasonic emulsification for 2 minutes;

[0042] (5) Mix the emulsion obtained in step 4 at 40 oC was stirred and volatilized at 800 rpm overnight, and then the ...

Embodiment 3

[0044] (1) Prepare 100 ml of hyaluronic acid aqueous solution with a mass / volume concentration of 0.75% at room temperature, add 0.1 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, stir well, 37 o Activation at C for 0.5 hours;

[0045] (2) Prepare 10 ml of gyrase solution with a mass / volume concentration of 1%, and add it dropwise to the activated hyaluronic acid solution, 37 o Stirring reaction at C for 4 hours, lyophilization after dialysis for 3 days;

[0046] (3) Dissolve the lyophilized hyaluronic acid in step 2 in 2 ml of water with a mass / volume concentration of 3%, add it to 50 ml of paraffin oil, add 1 ml of Span 80, and perform ultrasonic emulsification for 3 minutes;

[0047] (4) Add 0.5 ml of coumarin aqueous solution with a mass / volume concentration of 3% to the emulsion obtained in step 3, and continue ultrasonic emulsification for 5 minutes;

[0048] (5) Mix the emulsion obtained in step 4 at 40 o C was stirred and volatilized at 1500 rpm overnight, and ...

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Abstract

The invention discloses a method for preparing polysaccharide microspheres, and provides a means for cross-linking a polysaccharide microsphere material by a medicine. According to the method, a hyaluronic acid microsphere material with stable structure is obtained via the action of medicine cross-linking, and by taking hyaluronic acid as a matrix material and adopting an inverse emulsion cross-linking technology. According to the method disclosed by the invention, a toxic chemical cross-linking agent is avoided, the biological activity of hyaluronic acid and the embedded medicine can be reserved, and the use safety of the polysaccharide microsphere material can be remarkably improved. The method disclosed by the invention is simple and practicable in process and equipment, and safe to operate, has the advantages of being low in preparation temperature, fast in curing speed, short in treatment period and the like, and has an application value in the aspects of medicine controlled release and tissue engineering.

Description

technical field [0001] The invention belongs to the field of biomedical polymer materials, and in particular relates to a method for preparing polysaccharide microspheres by cross-linking drugs. Background technique [0002] Microsphere materials with biodegradable properties can be used for the embedding and release of drugs, proteins or genes, and have been widely used in research in the fields of drug controlled release, tissue engineering and regenerative medicine. Selecting the appropriate microsphere system can realize the slow release of the drug in the body and achieve the ideal therapeutic effect. In the field of tissue engineering and regenerative medicine, microspheres are often used to embed cell growth factors and genes, improve the biological activity of cell scaffolds, and increase the tissue induction ability of scaffolds in vivo. Natural polysaccharides have excellent biocompatibility and adjustable biodegradability, and are ideal matrix materials for the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K47/36A61K31/7056
Inventor 谈华平
Owner NANJING UNIV OF SCI & TECH