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G6 lectin extracted from traditional Chinese medicines and applications thereof in detoxification, pressure stabilization, blood vessel softening and complication resistance

A technology of lectin and traditional Chinese medicine, which is applied to cardiovascular system diseases, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as prone to complications, toxic and side effects cannot be ruled out, achieve high permeability, and improve adjuvant therapy effect, easy absorption effect

Inactive Publication Date: 2013-04-24
王学英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a traditional Chinese medicine that has the functions of detoxifying, stabilizing blood pressure, dredging blood vessels, and preventing complications for hypertensive patients who cannot eliminate the side effects of antihypertensive western medicines in the prior art and are prone to complications. Extraction of G6 lectin

Method used

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  • G6 lectin extracted from traditional Chinese medicines and applications thereof in detoxification, pressure stabilization, blood vessel softening and complication resistance
  • G6 lectin extracted from traditional Chinese medicines and applications thereof in detoxification, pressure stabilization, blood vessel softening and complication resistance
  • G6 lectin extracted from traditional Chinese medicines and applications thereof in detoxification, pressure stabilization, blood vessel softening and complication resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Preparation of G6 lectin

[0041] Weigh 50 grams each of Panax notoginseng, Eucommia, Pueraria root, Rehmannia glutinosa, Salvia miltiorrhiza, and kelp dry medicine, grind into 50-mesh fine powder, add 5 times the volume of Tris buffer (50mmol / L Tris-HCl, 1mmol / L MgCl 2 , 1mmol / L CaCl 2 , pH8.0), homogenate, and soak for 12 hours at 4°C. Centrifuge at 3000rpm for 15min at 4°C, and take the supernatant.

[0042] Filter the obtained supernatant by hypertonic agglutination method to filter out protein, starch, pectin, tannin and other macromolecular substances and microorganisms, take the filtrate, and use 50 times the volume of 0.5mol / L PBS with pH 7.4 at 4°C Dialyzed for 24 hours, concentrated and dried to obtain G6 lectin. The obtained protein was quantitatively analyzed, and the result was that 250 mg of G6 lectin protein was obtained from 300 g of Chinese patent medicine powder.

Embodiment 2

[0043] Example 2: Preparation of G6 lectin

[0044] Weigh 100 grams each of Panax notoginseng, Eucommia, Pueraria root, Rehmannia glutinosa, Salvia miltiorrhiza, and kelp dry medicine, grind into 100 mesh fine powder, add 5 times the volume of Tris buffer (50mmol / L Tris-HCl, 1mmol / LMgCl 2 , 1mmol / L CaCl 2 , pH8.0), homogenate, and soak for 12 hours at 4°C. Centrifuge at 3000rpm for 15min at 4°C, and take the supernatant.

[0045] Filter the obtained supernatant by hypertonic agglutination method to filter out protein, starch, pectin, tannin and other macromolecular substances and microorganisms, take the filtrate, and use 50 times the volume of 0.5mol / L PBS with pH 7.4 at 4°C Dialyzed for 24 hours, concentrated and dried to obtain G6 lectin. The obtained protein was quantitatively analyzed, and the result was that 550 mg of G6 lectin protein was obtained from 600 g of Chinese patent medicine powder.

Embodiment 3

[0046] Example 3: Preparation of G6 lectin

[0047] Weigh 70 grams each of Panax notoginseng, Eucommia, Pueraria root, Rehmannia glutinosa, Salvia miltiorrhiza, and kelp dry medicine, grind into 80-mesh fine powder, add 5 times the volume of Tris buffer (50mmol / L Tris-HCl, 1mmol / LMgCl 2 , 1mmol / L CaCl 2 , pH8.0), homogenate, and soak for 12 hours at 4°C. Centrifuge at 3000rpm for 15min at 4°C, and take the supernatant.

[0048] Filter the obtained supernatant by hypertonic agglutination method to filter out protein, starch, pectin, tannin and other macromolecular substances and microorganisms, take the filtrate, and use 50 times the volume of 0.5mol / L PBS with pH 7.4 at 4°C Dialyzed for 24 hours, concentrated and dried to obtain G6 lectin. Quantitative analysis of the obtained protein showed that 370 mg of G6 lectin protein was obtained from 420 g of Chinese patent medicine powder.

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Abstract

The invention discloses a G6 lectin extracted from the traditional Chinese medicines, namely pseudo-ginseng, eucommia ulmoides, radix puerariae, rehmannia, salvia miltiorrhiza and kelp. A preparation method of the lectin comprises the following steps of: 1) weighing dry pseudo-ginseng, eucommia ulmoides, radix puerariae, rehmannia, salvia miltiorrhiza and kelp according to the same weight, grinding thetraditional Chinese medicines into mixed medicine powder with the granularity of 50-100 meshes; 2) adding the obtained mixed medicine powder into a Tris buffer solution of which the volume is 5 times that of the mixed medicine powder, homogenizing, soaking for 12 hours at the temperature of 4DEG C, and centrifuging to obtain a supernatant; 3) filtering the obtained supernatant by a hypertonic agglutination method to remove impurities to obtain phytolectin-containing filter liquor; and 4) dialyzing, concentrating and drying the filter liquor to obtain the G6 lectin. The G6 lectin provided by the invention not only has the characteristics of the phytolectin, but also has an adjusting function for recovering a human sympathetic nervous system, so that the G6 lectin disclosed by the invention has a bran-new breakthrough for the stabilization of the hypertension and the improvement of the complication.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, in particular to a G6 lectin extracted from traditional Chinese medicines and its use in detoxification and blood pressure stabilization, softening of blood vessels and prevention of complications. Background technique [0002] Lectin is a glycoprotein or sugar-binding protein that can be synthesized and secreted by both animal cells and plant cells. It is named lectin because it can agglutinate red blood cells (including blood group substances). Commonly used are plant agglutinin (Phytoagglutin, PNA), usually named after the plant from which it is extracted, such as concanavalin A (Conconvalina, ConA), wheat germ agglutinin (Wheat germ agglutinin, WGA), peanut agglutinin (Peanut agglutinin, PNA) and soybean agglutinin (Soybean agglutinin, SBA), etc., lectin is their general term. Lectins play an important role in cell recognition and adhesion reactions, mainly promoting intercellula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/804A61K38/16A61P9/12
Inventor 王学英
Owner 王学英
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