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Method for extracting maize yellow pigment from maize protein

A technology of zeaxanthin and zein, applied in chemical instruments and methods, azo dyes, organic dyes, etc., to achieve the effects of easy industrial transformation, improved extraction rate, and simple process

Inactive Publication Date: 2013-05-01
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the above problems that the operation of extracting zeaxanthin from zein is complicated and it is difficult to realize industrial production, the present invention provides a high extraction rate, simple and easy to operate The method for extracting zeaxanthin from zein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Ultrafinely pulverize corn gluten powder into 300-mesh superfine powder, weigh 2g of zein superfine powder in the reaction device, add deionized water, control the mass concentration of superfine powder to 4%, and add 0.3% of superfine powder mass Xylanase with an enzyme activity of 15,000IU / g and Aspergillus oryzae protease with an enzyme activity of 60,000IU / g accounting for 0.8% of the ultrafine powder mass were enzymatically hydrolyzed at 42±5°C for 4 hours, the enzymatic hydrolyzate was centrifuged, and the supernatant was discarded , have to precipitate;

[0032] (2) Add ethanol to the precipitate in step (1), add 24mL of 90% ethanol according to the ratio of solid to liquid 1:12, and extract in a microwave reaction system with a microwave power of 220W, a time of 115s, and a temperature of 42±5°C. After the reaction is completed, centrifuge at a high speed of 10000r / min for 10min, and collect the supernatant;

[0033] (3) The supernatant was stored at -18°C ...

Embodiment 2

[0036] (1) Superfine corn gluten powder into 500 mesh superfine powder, weigh 2g of corn gluten superfine powder in the reaction device, add deionized water, control the mass concentration of superfine powder to 5%, add 0.5% of superfine powder mass Xylanase with an enzyme activity of 15,000IU / g and Aspergillus oryzae protease with an enzyme activity of 60,000IU / g accounting for 1.2% of the ultrafine powder mass were enzymatically hydrolyzed at 42±5°C for 5h, the enzymatic hydrolyzate was centrifuged, and the supernatant was discarded , have to precipitate;

[0037] (2) Add ethanol to the precipitate in step (1), add 30mL of 95% ethanol according to the ratio of solid to liquid 1:15, and extract in a microwave reaction system with a microwave power of 480W, a time of 115s, and a temperature of 42±5°C. After the reaction is completed, centrifuge at a high speed of 10000r / min for 10min, and collect the supernatant;

[0038] (3) The supernatant was stored at -18°C for 10 hours, ...

Embodiment 3

[0041] (1) Ultrafinely pulverize corn gluten powder into 400-mesh superfine powder, weigh 2g of zein superfine powder in the reaction device, add deionized water, control the mass concentration of superfine powder to 3%, and add 0.1% of superfine powder mass Xylanase with an enzyme activity of 15,000IU / g and Aspergillus oryzae protease with an enzyme activity of 60,000IU / g accounting for 0.3% of the ultrafine powder mass were enzymatically hydrolyzed at 42±5°C for 2 hours, the enzymatic hydrolyzate was centrifuged, and the supernatant was discarded , have to precipitate;

[0042] (2) Add ethanol to the precipitate in step (1), add 36mL of 92% ethanol according to the ratio of solid to liquid 1:18, and extract in a microwave reaction system with a microwave power of 360W, a time of 85s, and a temperature of 42±5°C. After the reaction is completed, centrifuge at a high speed of 10000r / min for 10min, and collect the supernatant;

[0043] (3) The supernatant was stored at -18°C f...

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Abstract

The invention relates to the technical field of corn gluten meal deep processing, in particular to a method for extracting maize yellow pigment from maize protein. The method comprises the following steps of performing superfine grinding on the corn gluten meal into ultra-fine powder of which the particle size is 300 to 500 meshes, adding water, xylanase and aspergillus oryzae protease into the ultra-fine powder to perform enzymolysis on the ultra-fine powder, centrifuging the enzymatic hydrolysate, and removing the supernate to obtain a precipitate; adding ethanol into the precipitate, extracting the mixture in a microwave reaction system, and collecting the supernate; and removing protein from the supernate, concentrating the filtrate under reduced pressure to obtain a paste, and freezing the paste under vacuum to dry the paste until the weight of the paste is consistent. The xylanase and the aspergillus oryzae protease are adopted to perform enzymolysis on the maize protein ultra-fine powder, so that the extraction rate of the maize yellow pigment is well improved; and the step of superfine grinding facilitates the dissolving of the maize yellow pigment in an extraction process.

Description

technical field [0001] The invention relates to the technical field of deep processing of corn gluten, in particular to a method for extracting zeaxanthin from corn gluten. Background technique [0002] Zeaxanthin is a mixture of several carotenoids, of which dihydroxycarotene accounts for about 81.2%, monohydroxycarotene accounts for 17.2%, and carotene accounts for about 1.6%. In 1962, Blessin confirmed that zeaxanthin exists in a complex form with protein in corn. Its content varies with different types of corn and protein content. The content of zeaxanthin in corn gluten powder reported at home and abroad is mostly between 300μg / g-700μg / g. Among them, multiple conjugated double bonds in hydroxycarotene make it have a bright color. Lutein is not a precursor of VA, and it cannot be transformed even after being absorbed by the intestinal tract, so it can maintain its own characteristics to the greatest extent. Therefore, most of the domestic processing of corn gluten me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
Inventor 陈相艳李晓玲孙华王文亮弓志青程安玮靳琼
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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