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Ammonia oxidizing bacteria and application thereof

A technology of ammonia oxidizing bacteria and nitrosomonas, applied in the field of environmental microorganisms, can solve the problems of restricted application, limited tolerance of ammonia nitrogen concentration, low ammonia oxidation rate, and low ammonia nitrogen removal efficiency, and achieve ammonia oxidation rate and ammonia nitrogen High removal efficiency and improved ammonia nitrogen removal rate

Active Publication Date: 2013-05-01
浙江至美环境科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Combining the ammonia oxidizing bacteria described in the above documents and patents, these strains show a certain efficiency of ammonia nitrogen removal in sewage biological nitrogen removal, but overall, the ammonia oxidation rate and ammonia nitrogen removal efficiency exhibited by these strains are still low In addition, the tolerance of these strains to the concentration of ammonia nitrogen is also very limited, and the current situation of a large amount of high-ammonia nitrogen sewage restricts the application of these strains

Method used

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  • Ammonia oxidizing bacteria and application thereof
  • Ammonia oxidizing bacteria and application thereof
  • Ammonia oxidizing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Isolation and Identification of Nitrosomonas CCTCC M2012456

[0048] (1) culture medium

[0049] Liquid medium: (NH 4 ) 2 SO 4 , 2.0g / L; KH 2 PO 4 , 0.7g / L; Na 2 HPO 4 , 7g / L; NaHCO 3 , 1g / L; MgSO 4 ·7H 2 O, 0.2g / L; CaCl 2 2H 2 O, 5mg / L; Fe-EDTA, 1mg / L; pH adjusted to 7.8-8.0, sterilized at 121°C for 20 minutes;

[0050] Solid medium: add 1.5-2% agar to the above liquid medium components.

[0051] (2) Isolation and purification of bacterial strains

[0052] Take Hangzhou Sewage Treatment Plant A 2 For the activated sludge in the aerobic section of the / O process, take 1mL of sludge for gradient dilution of 10 0 、10 1 、10 2 、10 3 、10 4 、10 5 After doubling, take 150-200 μL of the diluted solution and spread it on a solid medium plate, incubate at a constant temperature of 30°C in the dark for 1-2 weeks, and observe the growth of the colonies on the plate.

[0053] Pick a single colony and inoculate it into 50mL liquid medium, and culture it...

Embodiment 2

[0071] The expanded culture of embodiment 2 nitrosomonas CCTCC M2012456

[0072] (1) prepare 20L culture medium;

[0073] The medium components are: (NH 4 ) 2 SO 4 , 2.0g / L; KH 2 PO 4 , 0.7g / L; Na 2 HPO 4 , 7g / L; NaHCO 3 , 1g / L; MgSO 4 ·7H 2 O, 0.2g / L; CaCl 2 2H 2 O, 5mg / L; Fe-EDTA, 1mg / L; pH adjusted to 7.8-8.0, sterilized at 121°C for 20 minutes;

[0074] (2) The strain of the present invention enriched in shake flasks was transferred to a fermenter with 20 L of medium at an inoculum size of 5% for expanded cultivation.

[0075] The expanded culture conditions are: temperature 30°C, pH in the fermenter with NaHCO 3 Adjust to about 7.8, DO control in 1 ~ 2mg / L.

[0076] When the bacterial strain of the present invention is expanded to the sixth day, the initial ammonia nitrogen concentration in the culture medium is reduced from 200 mg / L to below 10 mg / L, and the removal rate is greater than 95%.

Embodiment 3

[0077] Example 3 Optimization of Nitrosomonas CCTCC M2012456 Best Growth Conditions for Removing Ammonia Nitrogen

[0078] Culture medium A:

[0079] K H 2 PO 4 , 0.7g / L; Na 2 HPO 4 , 7g / L; NaHCO 3 , 1g / L; MgSO 4 ·7H 2 O, 0.2g / L; CaCl 2 2H 2 O, 5mg / L; Fe-EDTA, 1mg / L;

[0080] Medium B:

[0081] (NH 4 ) 2 SO 4 , 2.0g / L; KH 2 PO 4 , 0.7g / L; Na 2 HPO 4 , 7g / L; NaHCO 3 , 1g / L; MgSO 4 ·7H 2 O, 0.2g / L; CaCl 2 2H 2 O, 5mg / L; Fe-EDTA, 1mg / L.

[0082] (1) The strain of the present invention is most suitable for NH 4 + -N concentration

[0083] The ammonia oxidation rate of ammonia oxidizing bacteria was investigated in the form of batch culture under different initial ammonia nitrogen concentrations.

[0084] Add culture solution A and 1mL activated and enriched bacterial solution to a 250mL Erlenmeyer flask, add ammonium sulfate to make NH 4 + -N concentrations were set to 0, 10, 20, 50, 100, 200, 400, 600, and 1000 mg / L in sequence, and the entire reaction...

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Abstract

The invention discloses an ammonia oxidizing bacteria and the application thereof. The ammonia oxidizing bacteria is named as (Nitrosomonas eutropha) CM-NRO14 and is preserved in the China Center for Type Culture Collection (CCTCC for short) of Wuhan University in Wuhan, China on Nov. 12, 2012, the preservation number is CCTCC No: M 2012456. The invention also provides the application of the Nitrosomonas eutropha CCTCC M 2012456 to saprobic ammonia removal, and further provides the application of the Nitrosomonas eutropha CCTCC M 2012456 to water eutrophication treatment. The ammonia oxidizing bacteria has high ammoxidation rate and ammonia removal efficiency and is suitable for high-ammonia nitrogen waste water treatment.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, in particular to an ammonia oxidizing bacterium and its application. Background technique [0002] Water is an important resource for human survival. With the development of our country's economy, the contradiction of insufficient water resources has become increasingly prominent, which has become a bottleneck restricting the economic development of many cities and seriously affecting the growth of the national economy. According to the 2011 China Environmental Status Bulletin, the ammonia nitrogen discharge in 2011 was 826,000 tons, which was 0.41% lower than that in 2010 (Ministry of Environmental Protection of the People's Republic of China, 2011). Main pollutant indicators of water systems and major lakes such as Dianchi Lake, Taihu Lake, and Chaohu Lake. The eutrophication of water bodies caused by the discharge of nitrogenous pollutants is still very serious, threatening human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/08C02F3/34C12R1/01
Inventor 谢柳杨岳平杨浩峰周俊利胡安辉张挺
Owner 浙江至美环境科技有限公司
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