Application of triangular silver nano-sheet in separation of single-stranded DNA
A nanosheet and triangular silver technology, applied in the field of DNA purification, can solve the problems of low separation efficiency, cumbersome and complicated operation methods, poor separation effect, etc., and achieve high separation efficiency, strong specific recognition ability, and good chemical stability. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] The sample to be extracted is a phosphate buffer at pH 7.4, which contains three types of single-stranded DNA, the sequences of which are:
[0030] DNA a: 5′-CCA AAT GAA GAT ACG TAG CAA ACG ACA GGT-3′;
[0031] DNA b: 5'-ATC GGT CAG TAA TCT TCA CGA ATA ACA CAA-3';
[0032] DNA c: 5'-AGT GCT TAT ACA GCA AGA CCA CGA AGT TAC-3'.
[0033] where the concentration is 10 -8 M.
[0034] Using triangular silver nanosheets to extract single-stranded DNA a, the steps are as follows: (1) Prepare triangular silver nanosheets by seed growth method
[0035] Preparation of silver seed solution: add 0.25mL 0.5mg / mL sodium polystyrene sulfonate aqueous solution and 0.3mL 10mM newly prepared sodium borohydride aqueous solution to 5mL 2.5mM sodium citrate aqueous solution, and mix well; Add 5mL of 0.5mM silver nitrate aqueous solution dropwise evenly at a speed of 1 / min, stir for 20 minutes, and let stand for 24 hours to obtain the silver seed solution.
[0036] Preparation of triangu...
Embodiment 2
[0047] The sample solution to be separated is a phosphate buffer at pH 7, which contains two kinds of single-stranded DNA, the sequences of which are:
[0048] DNA a: 5'-CCA AAT GAAGAT ACG TAGCAA ACG ACA GGT-3';
[0049] DNA b: 5'-ATC GGT CAG TAA TCT TCA CGA ATA ACA CAA-3'.
[0050] Among them, the concentration is DNA a 10 -11 M, DNA b 10 -8 M.
[0051] Using triangular silver nanosheets to separate and remove single-stranded DNA a, the steps are as follows:
[0052] (1) Preparation of triangular silver nanosheets by seed growth method
[0053] Preparation of silver seed solution: add 0.25mL 0.5mg / mL sodium polystyrene sulfonate aqueous solution and 0.3mL 10mM newly prepared sodium borohydride aqueous solution to 5mL 2.5mM sodium citrate aqueous solution, and mix well; Add 5mL of 0.5mM silver nitrate aqueous solution dropwise evenly at a speed of 1 / min, stir for 20 minutes, and let stand for 24 hours to obtain the silver seed solution.
[0054] Preparation of triangular...
Embodiment 3
[0063] The sample solution to be separated is a phosphate buffer at pH 9, which contains two kinds of single-stranded DNA, the sequences of which are:
[0064] DNA a: 5′-CCA AAT GAA GAT ACG TAG CAA ACG ACA GGT-3′;
[0065] DNA b: 5'-ATC GGT CAG TAA TCT TCA CGA ATA ACA CAA-3'.
[0066] Among them, the concentration is DNA a 10 -10 M, DNA b 10 -8 M.
[0067] Using triangular silver nanosheets to separate and purify single-stranded DNA a, the steps are as follows:
[0068] (1) Preparation of triangular silver nanosheets by seed growth method
[0069] Preparation of silver seed solution: add 0.25mL 0.5mg / mL sodium polystyrene sulfonate aqueous solution and 0.3mL 10mM newly prepared sodium borohydride aqueous solution to 5mL 2.5mM sodium citrate aqueous solution, and mix well; Add 5mL of 0.5mM silver nitrate aqueous solution dropwise evenly at a speed of 1 / min, stir for 20 minutes, and let stand for 24 hours to obtain the silver seed solution.
[0070] Preparation of triangul...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More