Method for quickly identifying hybrid of miscanthus floridulus with silvergrass through using SSR molecule markers

A technology of molecular markers and five-jointed awns, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of long identification time, rapid identification of unfavorable hybrids, and environmental impact of morphological traits. Wide distribution range, simple operation and good repeatability
CN103074440AInactive Publication Date: 2013-05-01HUNAN AGRICULTURAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUNAN AGRICULTURAL UNIV
Publication Date
2013-05-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for quickly identifying an interspecific distant hybrid of a herbaceous energy plant, namely miscanthus floridulus with silvergrass through using SSR (Simple Sequence Repeat) molecule markers. The method comprises the steps that tender leaves of the plant are selected, cleaned with distilled water, and then subjected to genome DNA (Deoxyribonucleic Acid) extraction by the conventional CTAB (Cetyl Trimethyl Ammonium Bromide) method; specific bands of the miscanthus floridulus and the silvergrass in various regions are analyzed respectively based on PCR (Polymerase Chain Reaction) and the SSR molecule markers; a pair of appropriate SSR markers is screened out; the markers generate one specific band (about 100bp) in the miscanthus floridulus, and generate the other specific band (about 123bp) in the silvergrass; and the markers show codominance in a filial generation of the miscanthus floridulus and the silvergrass. Therefore, the SSR markers can be used for molecule identification of the filial generation of the miscanthus floridulus and the silvergrass. With the method, the authenticity of the hybrid of the miscanthus floridulus with the silvergrass can be identified quickly and precisely. The method for identifying the hybrid of the miscanthus floridulus with the silvergrass is simple to operate, quick, accurate, good in repeatability, and free from an influence of an environmental factor.
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Description

technical field

[0001] The invention belongs to the technical field of identification of plant hybrids, in particular to a molecular identification method of Miscanthus plant hybrids. Background technique

[0002] Miscanthus is a tall perennial herbaceous C4 plant in the Poaceae family. As the energy plant with the most development potential, it has attracted increasing attention. China is the origin and distribution center of Miscanthus, with rich wild resources and many ecological types. Miscanth plants have the characteristics of wide adaptability, strong stress resistance, high yield, high net energy output, good biomass quality, wide conversion and utilization channels, and good ecological value. They are suitable for planting and utilization on marginal land. Ecologically and economically efficient herbal energy plants. Grass (Miscanthus Sacchariflorus) in the genus Miscanthus is distributed in the northeast, northwest, north and east of China. It has strong stress r...

Claims

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