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Preparation method for wheat leaf stomata guard cell observation samples

A technology for guarding cells and leaves, applied in the field of plant research, can solve the problems of poor wheat leaves and other problems, and achieve the effect of good stomatal authenticity, efficient technical methods, and easy operation

Inactive Publication Date: 2013-05-01
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, these currently known schemes are not effective in wheat leaf samples

Method used

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  • Preparation method for wheat leaf stomata guard cell observation samples
  • Preparation method for wheat leaf stomata guard cell observation samples
  • Preparation method for wheat leaf stomata guard cell observation samples

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] Materials and Methods

[0018] 1. Experimental Materials

[0019] The test materials are wheat pollen plants obtained by anther in vitro culture technology. During the anther culture process, no doubling treatment was performed, and the pollen plants have both haploid and diploid. All the pollen plants were planted in the experimental field of Zhongpu Plant of the Chinese Academy of Agricultural Sciences, and the pollen plants were sampled and marked before plant jointing.

[0020] 2 Experimental methods

[0021] 2.1 Solution preparation

[0022] Fixative:

[0023] Ethanol / Acetic Acid Mixture: Mix 6 parts of absolute ethanol with 1 part of glacial acetic acid for sample fixation.

[0024] Clearing agent:

[0025] 10% potassium hydroxide solution: 1 gram of potassium hydroxide is dissolved in 10 ml of distilled water.

[0026] Glycerin solution: 1 ml of glycerin was dissolved in 2 ml of distilled water.

[0027] Saturated chloral hydrate solution: 4 g of chloral ...

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Abstract

The invention discloses a preparation method for wheat leaf stomata guard cell observation samples, and relates to the plant research technology. The method comprises the steps of preparation of a material to be treated, fixation of the material to be treated, dehydration and whole transparency. The method is characterized in that a transparent reagent adopted by the whole transparency is obtained by mixing chloral hydrate, distilled water and glycerol according to the weight ratio of 4 g: 1 ml: (0-0.5) ml (the ratio can be rationally widened to a range). For preparing the wheat leaf stomata guard cell observation samples, clear microscopic observation images are obtained.

Description

technical field [0001] The invention relates to plant research technology, in particular to a preparation method for observation samples of stomatal guard cells of wheat leaves. Background technique [0002] The stomata of wheat leaves are composed of two dumbbell-shaped guard cells. Studies have shown that the length of the stomatal guard cells can be used as an indicator for identifying chromosomal ploidy of wheat plants (Wang Pei et al., 1989), and according to the condition of irrigated land and dry land The difference in length of stomatal guard cells at the tip of the flag leaf can preliminarily determine the drought tolerance of the genotype (Fan Guangnian et al., 1990, 1991). Because the observation of stomatal guard cells is simple, rapid and low-cost, it plays an important role in the indirect identification of chromosome ploidy and genotype drought tolerance. [0003] The samples for observation of stomatal guard cells in wheat leaves are generally prepared by di...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 赵林姝刘录祥古佳玉郭会君李军辉谢永盾赵世荣
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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