Method for promoting germination of elaeagnus mollis seed

A technology of Samara oil tree and seeds, which is applied in the field of promoting germination of Samara oil tree seeds, can solve the problems of high equipment requirements, complex process, chemical residues, etc., and achieve the effect of high safety, simple process operation, and promotion of germination

Inactive Publication Date: 2014-08-13
山西师范大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of low germination rate, high equipment requirements, complex process and easy chemical residue and seed mutation in the existing methods for promoting the germination of Samara oil tree seeds, the present invention provides a method for promoting the germination of Samara oil tree seeds. germination method

Method used

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  • Method for promoting germination of elaeagnus mollis seed

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Experimental program
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Effect test

Embodiment 1

[0034] A method for promoting the germination of samara oil tree seeds, comprising the following steps:

[0035] (1) Remove the membranous exocarp of Samara oleifera seeds, freeze in a -20°C refrigerator for 24 hours, and then refrigerate in a 4°C refrigerator for 7 days;

[0036] (2) Select plump and disease-free Samara oil tree seeds, soak them in hot water at 45°C for 40 minutes, and peel off the hard mesocarp;

[0037] (3) Soak the seeds of step (2) with 10 times diluted Bacillus subtilis QM34 bacterial solution for 1 day, rinse with sterile water, peel off the endocarp and wait for seeding;

[0038] (4) Sow the seeds of step (3) with the embryo end facing down in the cultivation medium with a mass ratio of flower nutrient soil: vermiculite of 1:1, place at 25°C, humidity 60%, and light-dark cycle 12h / 12h constant temperature light incubator for germination.

Embodiment 2

[0040] A method for promoting the germination of samara oil tree seeds, comprising the following steps:

[0041] (1) Remove the membranous exocarp of Samara oleifera seeds, freeze in a -20°C refrigerator for 26 hours, and then refrigerate in a 4°C refrigerator for 7 days;

[0042] (2) Select plump and disease-free Samara oil tree seeds, soak them in hot water at 55°C for 35 minutes, and peel off the hard mesocarp;

[0043] (3) Soak the seeds of step (2) with 50 times diluted Bacillus subtilis QM34 bacterial solution for 1 day, rinse with sterile water, peel off the endocarp and wait for seeding;

[0044] (4) Sow the seeds of step (3) with the embryo end facing down in the cultivation medium with a mass ratio of flower nutrient soil: vermiculite of 1:1, place at 25°C, humidity 60%, and light-dark cycle 12h / 12h constant temperature light incubator for germination.

[0045] Among them, Bacillus subtilis QM34 was cultured with 100 mL of beef extract peptone at 170 r / min, shake ...

Embodiment 3

[0047] A method for promoting the germination of samara oil tree seeds, comprising the following steps:

[0048] (1) Remove the membranous exocarp of Samara oleifera seeds, freeze in a -20°C refrigerator for 28 hours, and then refrigerate in a 4°C refrigerator for 7 days;

[0049] (2) Select plump and disease-free Samara oil tree seeds, soak them in hot water at 50°C for 45 minutes, and peel off the hard mesocarp;

[0050] (3) Soak the seeds of step (2) with 100 times diluted Bacillus subtilis QM34 bacterial solution for 1 day, rinse with sterile water, peel off the endocarp and wait for seeding;

[0051] (4) Sow the seeds of step (3) with the embryo end facing down in the cultivation medium with a mass ratio of flower nutrient soil: vermiculite of 1:1, place at 25°C, humidity 60%, and light-dark cycle 12h / 12h constant temperature light incubator for germination.

[0052] Among them, Bacillus subtilis QM34 was cultured with 100 mL of beef extract peptone at 170 r / min, shake t...

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Abstract

The invention discloses a method for promoting germination of elaeagnus mollis seed, belonging to the technical field of germination of plant seeds. According to the invention, the problems of low germination rate, high requirements for equipment and complicated process existed in the existing technology can be solved. The method for promoting germination of elaeagnus mollis seed disclosed by the invention comprises the following steps: removing membranous and external peel of seeds, putting the seeds in a refrigerator at the temperature of 20 DEG C below zero and freezing the seeds for 24-28 hours, then refrigerating the seeds in the refrigerator at the temperature of 4 DEG C for 7 days; soaking the seeds in hot water at the temperature of 45-55 DEG C for 35-45 minutes and peeling hard mesocarp; soaking the seeds for 1 day by using a bacillus subtilis QM 34 bacteria liquor which is diluted by 1-100 times, cleanly washing the seeds by using aquae sterilisata and peeling endocarp for seeding; and seeding the seeds in a culture medium with flower nutritional soil and vermiculite in a mass ratio of 1:1 by facing embryo ends downwards and putting the culture medium in a constant-temperature illumination culture box at the temperature of 25 DEG C, a humidity of 65% and a light-dark cycle of 12 hours / 12hours to germinate. The method for promoting germination of elaeagnus mollis seed disclosed by the invention has the advantages of high safety, good sustainability, simple process operation, good repeatability and high germination rate.

Description

technical field [0001] The invention belongs to the technical field of plant seed germination, and relates to a method for promoting seed germination of Samara oil tree. Background technique [0002] Samara ( Elaeagnus mollis diele) is a member of the genus Elaeagnaceae (Elaeagnaceae) Elaeagnu s) Plant is a perennial deciduous shrub or tree, which is only distributed in Shanxi Xiangning, Yixian and some parts of Shaanxi in China, especially in the southern end of Shanxi Luliang Mountain and the western part of Zhongtiao Mountain. It is a unique tree species in my country , which plays an important role in biodiversity conservation. [0003] In the "List of National Key Protected Wild Plants (First Batch)" published by the State Council on August 4, 1999, Samara oil tree was listed as a national second-class rare and endangered plant. The main reasons for endangerment are short seed life, low germination rate, special fruit shape and structure, which is not conducive to pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02
Inventor 胡青平徐建国李玲常笑陈春萍边彩峰
Owner 山西师范大学
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