Method and primer for detecting necrosis virus of goldfish hematopoietic organ by loop-mediated isothermal amplification

A technology of hematopoietic organ necrosis and loop-mediated isothermal, applied in the field of warm amplification detection, can solve the problem that there is no detection method and related primers for goldfish hematopoietic organ necrosis virus LAMP, and achieve simple operation, strong specificity and good sensitivity Effect

Inactive Publication Date: 2015-07-01
SHENZHEN AUDAQUE DATA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The core of this amplification method lies in the ingenious design of primers. At present, there is no LAMP detection method and related primers for goldfish hematopoietic organ necrosis virus.

Method used

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  • Method and primer for detecting necrosis virus of goldfish hematopoietic organ by loop-mediated isothermal amplification
  • Method and primer for detecting necrosis virus of goldfish hematopoietic organ by loop-mediated isothermal amplification
  • Method and primer for detecting necrosis virus of goldfish hematopoietic organ by loop-mediated isothermal amplification

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Experimental program
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Effect test

Embodiment

[0035] 1. Materials and methods

[0036] 1. Virus suspension

[0037] The virus suspension used in the experiment in this case includes goldfish hematopoietic organ necrosis virus (abbreviated as GFHNV), Koi herpesvirus (KHV), channel catfish virus (CCV), and rhizome iridescent virus (Bohle iridovirus, BIV), spring viraemia of carp virus (SVCV), Yellowtail asictes verus (YAV), Viral nervous necrosis virus (VNNV), Infectious salmon anemia virus (ISAV), Infectious pancreatic necrosis virus (IPNV), Viral haemorrhagic septicemia virus (VHSV), Infectious hematopoietic necrosis virus (Infectious hematopoietic necrosis virus, IHNV), flounder rhabdovirus (Hirame rhabdovirus, HRV), strain 9701 belongs to soft-shelled turtle iridovirus (STIV), strains 9506 and 9507 belong to the genus Ranavirus of the family Iridoviridae strains. The above virus suspensions are all preserved by the Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry-Exit Inspection and Quar...

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Abstract

The invention relates to the field of nucleic acid detection, especially disclosing a primer for detecting necrosis virus of goldfish hematopoietic organ by loop-mediated isothermal amplification, a kit based on the primer and a method for detecting necrosis virus of goldfish hematopoietic organ by loop-mediated isothermal amplification. The primer disclosed by the invention is selected from at least one group of 1-5 primer groups designed according to polymerase gene DNA of the necrosis virus of goldfish hematopoietic organ, helicase gene, terminase gene and triplex protein gene. The method and primer for detecting necrosis virus of goldfish hematopoietic organ by loop-mediated isothermal amplification disclosed by the invention provide a new detection method of good sensitivity and strong specificity for necrosis virus of goldfish hematopoietic organ, which can be completed just at constant temperature, is simply operated and specially suitable for inspection and quarantine departments as well as field inspection.

Description

technical field [0001] This application relates to the field of nucleic acid molecular detection, in particular to a primer for the detection of goldfish hematopoietic organ necrosis virus loop-mediated isothermal amplification, and a non-diagnostic goldfish hematopoietic organ necrosis virus loop-mediated isothermal amplification based on the primer. Amplified detection method. Background technique [0002] Goldfish Haematopoietic Necrosis Virus (GFHNV for short) is also known as Herpsviral Haematopoietic Necrosis Virus (HVHNV), because the disease is the second herpes virus isolated from carps. According to the systematic naming rules of the International Committee on Systematic Taxonomy of Viruses, it is officially named Cyprinid herpescirus 2 (CyHV-2). Due to the lack of research on the disease and the lack of relevant quarantine systems, the international trade of ornamental goldfish has accelerated the spread of the disease worldwide, causing destructive effects on th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
Inventor 何俊强郑晓聪贾鹏史秀杰刘荭兰文升王津津于力
Owner SHENZHEN AUDAQUE DATA TECH
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