Method for determining content of hesperidin in Citrus Bioflavonoids
A technology of citrus bioflavonoids and determination method, which is applied in the field of biological testing, can solve the problems of inability to remove impurities, complicated treatment, long pretreatment time, etc., and achieve the effect of simple operation, good specificity and repeatability, and high recovery rate
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[0017] (1) Preparation of diluted solution: Take 20mmol / l sodium hydroxide solution and 50% methanol solution and mix evenly at a ratio of 0.5-1.5:1.5-0.5; (2) Preparation of reference solution: Accurately weigh orange peel Hesperidin and naringin reference substance amount, add dilute solution to make the reference substance solution whose concentration of hesperidin and naringin are both 0.10mg / ml; (3), preparation of test solution: accurately weigh 10-15mg Put the sample in a 100ml volumetric flask, add 80ml of diluting solution, ultrasonicate at 60-70°C for 10-20min, take it out, cool to room temperature, and dilute to the mark with diluting solution. Take a part of the solution and filter it with a 0.45 μm microporous membrane for later use; (4) Determine the chromatographic conditions: Chromatographic column: C18 chromatographic column, 4.6mm×250mm, 5 μm; mobile phase and gradient: use a 0.45 μm microporous membrane for the mobile phase After filtering, degas; mobile pha...
Embodiment 1
[0019] Example 1, citrus bioflavonoid mixture Batch number: 20110918
[0020] Taking hesperidin as the content determination index, the content determination is carried out as follows:
[0021] (1) Preparation of diluted solution: add 20mmol / l sodium hydroxide solution and 50% methanol solution and mix evenly in a ratio of (1:1);
[0022] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of hesperidin and naringin reference substances, add a diluted solution to prepare a reference solution with a concentration of 0.10 mg / ml of hesperidin and naringin;
[0023] (3) Preparation of the test solution: Accurately weigh 12.5mg of sample into a 100ml volumetric flask, add 80ml of diluent solution, ultrasonicate at 65°C for 15min, take it out, cool to room temperature, and dilute to the mark with dilute solution. Take part of the solution and filter it with a 0.45 μm microporous membrane for later use;
[0024] (4) Determine the chromatographic ...
Embodiment 2
[0027] Example 2, citrus bioflavonoid mixture Batch number: 20120107
[0028] Taking hesperidin as the content determination index, the content determination is carried out as follows:
[0029] (1) Preparation of diluted solution: add 20mmol / l sodium hydroxide solution and 50% methanol solution and mix evenly in a ratio of (1.5:0.5);
[0030](2) Preparation of reference substance solution: Accurately weigh an appropriate amount of hesperidin and naringin reference substances, add a diluted solution to prepare a reference solution with a concentration of 0.10 mg / ml of hesperidin and naringin;
[0031] (3) Preparation of the test solution: Accurately weigh 10.5mg of the sample into a 100ml volumetric flask, add 80ml of the diluted solution, ultrasonicate at 60°C for 10min, take it out, cool to room temperature, and dilute to the mark with the diluted solution. Take part of the solution and filter it with a 0.45 μm microporous membrane for later use;
[0032] (4) Determine the ...
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