Unlock instant, AI-driven research and patent intelligence for your innovation.

Microbial culture medium prepared by using TCM (traditional Chinese medicine) waste residues, as well as applications and preparation method thereof

A technology for microbial culture medium and traditional Chinese medicine waste residues, applied in biochemical equipment and methods, botanical equipment and methods, microorganisms, etc., can solve problems such as differences in processing technology, loss of nutrients, and retention, and reduce production costs. Pollution problem, the effect of full utilization

Inactive Publication Date: 2014-07-09
CHENGDU QIANKUN VETERINARY PHARMA +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is widely used, but has certain limitations, such as: 1. The sources of traditional Chinese medicine waste residues are different, the formula is complicated, the processing technology is different, and the active ingredients are different. It is difficult to make an accurate evaluation and evaluation of the traditional Chinese medicine waste residue Unified quality standards; 2. Traditional Chinese medicine waste residues are generally dried and crushed and added directly to the feed, but because Chinese medicine waste residues contain a large amount of crude fiber that is not easy for animals to digest and absorb, resulting in the loss of its nutrients; 3. Due to the traditional Chinese medicine waste residue The content of medicinal active ingredients in the feed is low, and its addition in production is high, which often affects the palatability of the feed; 4. When manufacturers formulate Chinese herbal medicine additives, most of them are based on traditional Chinese medicine data or the use of Chinese herbal medicine. and functions, rather than the needs of different animal characteristics and growth stages, one-sided pursuit of the role and effect of addition, lack of scientific and systematic theoretical evidence; 5. At present, most of the evaluation of the utilization value of traditional Chinese medicine waste residues only stays at the apparent level Production performance, and the relationship between production performance and active ingredients of medicinal dregs is not clear, which limits the deeper processing and utilization of medicinal dregs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial culture medium prepared by using TCM (traditional Chinese medicine) waste residues, as well as applications and preparation method thereof
  • Microbial culture medium prepared by using TCM (traditional Chinese medicine) waste residues, as well as applications and preparation method thereof
  • Microbial culture medium prepared by using TCM (traditional Chinese medicine) waste residues, as well as applications and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1——determination of mycoplasma polysaccharide content

[0042] The culture medium obtained in comparative examples 1-9 is used to cultivate Cordyceps militaris and Cordyceps sinensis respectively, and the culture medium obtained in each comparative example sets five experiments of repeatedly cultivating Cordyceps militaris and Cordyceps sinensis, and the culture method is to adopt the Cordyceps militaris strain liquid and Cordyceps fungus seed liquid were spread on the surface of the culture medium obtained in Comparative Example 1-9 respectively, and the obtained culture was based on 25-27 ℃ constant temperature and dark culture for 10-15 days, and after sampling each culture medium from the fifth day The obtained sample was dried and the polysaccharide content of the medicinal fungus was determined. The experimental conditions were as follows: the concentrated sulfuric acid-phenol method was used to detect the polysaccharide content, and the standard curve w...

Embodiment 2

[0043] Embodiment 2——Determination of content of mycoplasma cordycepin

[0044] Comparative example 1-9 gained substratum is used for cultivating Cordyceps militaris respectively, and the experiment of five repeated cultivation Cordyceps militaris is arranged in each contrasting example gained substratum, and culture method is to adopt that Cordyceps militaris seed liquid is scattered in contrasting example respectively 1-9 The surface of the obtained medium, and the obtained culture is based on constant temperature and dark culture at 25-27°C for 10-15 days. From the fifth day, each medium is sampled, and the obtained samples are dried and treated with the medicinal fungus Cordyceps sinensis Cordycepin content determination experiment, the experimental conditions are as follows: use high performance liquid chromatography to detect the content of cordycepin, the chromatographic conditions are Diamonsil C18 column (4.6mm*150mm, 5μm), with phosphate buffer (pH6.5) (take 0.01mol / ...

Embodiment 3

[0045] Embodiment 3 - the cultivation degree of culture medium of the present invention to different fungi

[0046] Utilize the experimental condition of embodiment 1 and experimental method that control example 4-9 is respectively used to cultivate Cordyceps militaris, saccharomyces, Staphylococcus aureus and detects the 7th day, the 9th day and the 11th day of the microorganism of each culture. The polysaccharide content was calculated as the average value, and the obtained results are shown in Table 4.

[0047] All the strains used above can be purchased in the market, or obtained from China Microorganism Culture Collection Center.

[0048] Table I

[0049]

[0050] Table II

[0051]

[0052] Table three

[0053]

[0054] Table four

[0055]

[0056] As can be seen from the experimental data in Table 1 and Table 2, when the culture medium of the present invention is cultivating microorganisms, the polysaccharide content in the fungal substance first increase...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a microbial culture medium, as well as applications and a preparation method thereof, and particularly relates to a microbial culture medium prepared by using TCM (traditional Chinese medicine) waste residues, as well as applications and a preparation method thereof. The microbial culture medium prepared by using TCM waste residues is composed of TCM schisandra extract waste residues and water, wherein the weight ratio of the TCM schisandra extract waste residues to the water is 1: (1-2). The microbial culture medium prepared by using TCM waste residues disclosed by the invention can effectively and fully utilize nutritional ingredients and active ingredients contained in TCM waste residues, and the production cost is low, the TCM waste residues are effectively and fully utilized, and the pollution to the environment is greatly reduced.

Description

technical field [0001] The invention relates to a microbial culture medium and its application and preparation method, in particular to a microbial culture medium prepared from traditional Chinese medicine waste residues and its application and preparation method. Background technique [0002] The Chinese herbal medicine industry is one of the traditional industries in China. With the development of the Chinese medicine industry in recent years, the application of Chinese herbal medicines and proprietary Chinese medicines has been increasing, which has brought about the pollution of Chinese medicine waste residues produced after the extraction of a large number of Chinese medicinal materials. The extraction process of existing Chinese medicinal materials is relatively simple, resulting in the waste residue of Chinese medicine containing a large amount of nutrients such as crude fiber, crude protein, crude fat, carbohydrates and amino acids, and the waste residue of Chinese me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00A01G1/04
Inventor 李英伦贺晓玉罗杰何晓利叶刚施飞房春林
Owner CHENGDU QIANKUN VETERINARY PHARMA