A kind of serum-free culture medium of fat stem cells and preparation method thereof
A serum-free medium, adipose stem cell technology, applied in the field of stem cell culture, can solve the problems of difficult standardization, unstable quality, unclear components, etc., to reduce the probability of bacterial contamination, improve the cell collection rate, and prolong the medium exchange time. Effect
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Embodiment 1
[0027] Embodiment 1 A kind of serum-free culture medium of fat stem cells
[0028] The adipose stem cell serum-free medium includes basal medium, β globulin, L-arginine, bactericide, type III collagen and cell attachment factor; the basal medium is DMEM / F12 medium, and the The final concentration of beta globulin is 14 μg / mL, the final concentration of the L-arginine is 80 mg / mL, the final concentration of the fungicide is 60 μg / mL, and the final concentration of the type III collagen is 4 μg / mL , the final concentration of the cell attachment factor is 25 μg / mL; the fungicide is composed of aloe vera tincture and ethyl allicin in a mass ratio of 5:2; the cell attachment factor is composed of polylysine and laminin The protein is composed at a mass ratio of 8:5.
[0029] The preparation method of the adipose stem cell serum-free medium is:
[0030] S1. Add L-arginine and β-globulin to the basal medium, and stir at 200 rpm for 15 minutes until evenly stirred to obtain mixture...
Embodiment 2
[0033] Embodiment 2 A kind of adipose stem cell serum-free culture medium
[0034] The adipose stem cell serum-free medium includes basal medium, β globulin, L-arginine, bactericide, type III collagen and cell attachment factor; the basal medium is DMEM / F12 medium, and the The final concentration of beta globulin is 18 μg / mL, the final concentration of the L-arginine is 100 mg / mL, the final concentration of the fungicide is 90 μg / mL, and the final concentration of the type III collagen is 8 μg / mL , the final concentration of the cell attachment factor is 35 μg / mL; the bactericide is composed of aloe vera tincture and ethyl allicin in a mass ratio of 7:3; the cell attachment factor is composed of polylysine and laminin The protein is composed in a mass ratio of 10:7.
[0035] The preparation method of the adipose stem cell serum-free medium is:
[0036] S1. Add L-arginine and β-globulin to the basal medium, and stir at 300 rpm for 20 minutes until evenly stirred to obtain mix...
Embodiment 3
[0039] Embodiment 3 A kind of adipose stem cell serum-free culture medium
[0040]The adipose stem cell serum-free medium includes basal medium, β globulin, L-arginine, bactericide, type III collagen and cell attachment factor; the final concentration of the β globulin is 16 μg / mL, so The final concentration of the L-arginine is 90 mg / mL, the final concentration of the bactericide is 75 μg / mL, the final concentration of the type III collagen is 6 μg / mL, and the final concentration of the cell attachment factor is 30 μg / mL; the bactericide is composed of aloe tincture and ethyl allicin in a mass ratio of 6:2.5; the cell attachment factor is composed of polylysine and laminin in a mass ratio of 9:6.
[0041] The preparation method of the adipose stem cell serum-free medium is:
[0042] S1. Add L-arginine and β-globulin to the basal medium, and stir at a speed of 250 rpm for 18 minutes until the mixture is evenly stirred to obtain mixture I;
[0043] S2. Add type III collagen ...
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