DNA chip based cipher system
A DNA chip and encryption system technology, applied in the field of DNA chip-based encryption system, can solve the problems of high operation cost, high cost, and error-prone decryption, and achieve the effect of clear information transmission, cost saving, and cost increase
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0023] Example 1 Fabrication of Random Sequence DNA Chip
[0024] First, obtain a random DNA sequence. A random sequence composed of 0 and 1 numbers can be generated by a random sequence generator, and then press "00=A, 01=T, 10=G, 11=C" to convert into a random DNA sequence composed of "ATGC".
[0025] Then, the random DNA sequence generated above is screened. DNA sequences with GC content greater than 60% and less than 40%, and DNA sequences with the same letter A, T, G, and C, or with more than 8 consecutive repeated AT and GC DNA sequences are excluded.
[0026] Third, the DNA sequence of about 1-2 kb obtained above is sent to a DNA synthesizer to artificially synthesize DNA fragments.
[0027] Fourth, make a DNA chip dot matrix distribution map M, and use the Excel file format to compile the DNA sequence fragment name and sequence (G(i,j)) corresponding to each dot matrix.
[0028] Finally, through the DNA chip production system, the above-mentioned random sequence DNA fragments ...
Example Embodiment
[0029] Example 2 Making a random sequence DNA oligonucleotide probe
[0030] First, for each random DNA sequence G(i,j), a 15bp long DNA sequence S(i,j) is randomly intercepted, and the BLAST program is used for all the DNA sequences G(i,j) on the DNA chip with random sequences. Search and alignment, if it is found that the selected S(i,j) is the only one with 100% homology and less than 40% homology with other sequences, then the S(i,j) can be used to synthesize DNA oligonuclei Nucleotide probe. Each G(i,j) can select multiple S(i,j) to synthesize multiple oligonucleotide probes.
[0031] Second, the selected random DNA sequence fragment S(i,j) is synthesized with a DNA synthesizer with its reverse complementary sequence, and each S(i,j) is labeled with a different fluorescent dye, such as Cy3 or Cy5 mark.
[0032] Third, the synthesized oligonucleotide probe is purified by PAGE and stored in a centrifuge tube in powder form. The synthesis amount can be adjusted as needed. If 5...
Example Embodiment
[0034] Embodiment 3 Information Encryption Method
[0035] First, the processing of plaintext information. According to the agreement of the communication parties, the plaintext information can be transformed into a binary form, Braille code or Morse code form, etc. It is not necessary to perform conversion.
[0036] Then, the plaintext information processed above is marked on the DNA chip dot matrix distribution map M in the form of a 2-dimensional dot matrix. The process is similar to "writing" on the DNA chip dot matrix distribution map M, and "write" the above-mentioned plain text information on the DNA chip dot matrix distribution map M.
[0037] Third, the selected and labeled dot matrix above corresponds to the corresponding random DNA sequence G(i,j). According to its marked color and corresponding G(i,j), take out the corresponding oligonucleotide probe S(i,j) from the synthetic oligonucleotide probe library. Since each G(i,j) corresponds to multiple oligonucleotide prob...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap