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DNA chip based cipher system

A DNA chip and encryption system technology, applied in the field of DNA chip-based encryption system, can solve the problems of high operation cost, high cost, and error-prone decryption, and achieve the effect of clear information transmission, cost saving, and cost increase

Inactive Publication Date: 2013-05-22
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the most obvious disadvantage of their method is the lack of operability or the high cost of operation
For example, every message encryption or decryption process must make a DNA chip, which is costly and extremely low in information transmission efficiency
Their method is also difficult to solve the interference problem of some false positives in the chip hybridization process, making decryption prone to errors
Therefore, these DNA code system schemes are only a kind of imagination, and they are far from entering the practical stage.

Method used

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Experimental program
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Embodiment 1

[0023] The making of embodiment 1 random sequence DNA chip

[0024] First, a random DNA sequence is obtained. A random sequence composed of 0 and 1 numbers can be generated by a random sequence generator, and then converted into a random DNA sequence composed of "ATGC" according to "00=A, 01=T, 10=G, 11=C".

[0025] Then, the random DNA sequences generated above are screened. DNA sequences with GC content greater than 60% and less than 40% and DNA sequences with the same letters of A, T, G, and C or AT and GC repeated more than 8 times were excluded.

[0026] Thirdly, send the DNA sequence of about 1-2 kb obtained above to a DNA synthesizer to artificially synthesize DNA fragments.

[0027] Fourth, make a DNA chip dot matrix distribution map M, and compile the DNA sequence fragment name and sequence (G(i, j)) corresponding to each dot matrix in Excel file format.

[0028] Finally, through the DNA chip production system, the random sequence DNA fragments were microprinted on...

Embodiment 2

[0029] Embodiment 2 makes random sequence DNA oligonucleotide probe

[0030] First, for each random DNA sequence G(i, j), randomly intercept a 15bp long DNA sequence S(i, j), and use the BLAST program to analyze all DNA sequences G(i, j) on the random sequence DNA chip Search and compare, if it is found that the selected S(i, j) is the only one with 100% homology and less than 40% homology with other sequences, then the S(i, j) can be used to synthesize DNA oligonuclei nucleotide probe. Each G(i, j) can select multiple S(i, j) for synthesizing multiple copies of oligonucleotide probes.

[0031] Second, the selected random DNA sequence fragment S(i, j) is synthesized with a DNA synthesizer using its reverse complementary sequence, and each S(i, j) is labeled with a different fluorescent dye, such as Cy3 or Cy5-labeled.

[0032] Third, the synthesized oligonucleotide probes were purified by PAGE and stored in a centrifuge tube in powder form. The amount of synthesis can be a...

Embodiment 3

[0034] Embodiment 3 information encryption method

[0035] First, the processing of plaintext information. According to the agreement of the communication parties, the plaintext information can undergo various transformations, such as conversion into binary form, Braille code or Morse code form, etc. It is also possible not to perform conversion.

[0036]Then, the above-mentioned processed plaintext information is marked on the DNA chip lattice distribution map M in the form of a 2-dimensional lattice. The process is similar to "writing" on the dot matrix map M of the DNA chip, "writing" the above-mentioned plaintext information on the dot matrix map M of the DNA chip.

[0037] Third, the above-mentioned selected and labeled lattices correspond to the corresponding random DNA sequences G(i, j). According to its marked color and corresponding G(i, j), the corresponding oligonucleotide probe S(i, j) is taken out from the synthesized oligonucleotide probe library. Since each ...

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Abstract

The invention provides a DNA chip based cipher system. The DNA of the DNA chip of the cipher system is a random sequence G (i, j). Oligonucleotide probes S (i, j) are oligonucleotide fragments obtained through artificially synthesizing the reverse complement sequences of a small-segment DNA sequence randomly intercepted from the random DNA sequence G (i, j) corresponding to the lattices of the DNA chip, and the uniqueness of the small-segment DNA sequence in the DNA chip is determined through using BLAST. Information encryption is that a DNA chip lattice distribution map M is marked with different G (i, j) to represent plaintext information, and then the oligonucleotide probes S (i, j) corresponding to the G (i, j) are mixed; and information decryption is that the oligonucleotide probes S (i, j) are hybridized with the random sequence DNA sequence. The encryption of same plaintext information can be realized through a large amount of different S (i, j) probe mixture combinations and can be admixed with any unlabeled DNA at will. The S (i, j) probes are composed of the random DNA sequence, and plaintext information related information is not coded in the DNA sequences of the mixtures of the S (i, j) probes, so the system cannot be decoded.

Description

technical field [0001] The invention belongs to the field of information security, in particular to a cryptographic system based on a DNA chip. Background technique [0002] DNA chip technology refers to the in situ synthesis of oligonucleotides (called DNA chip) on a solid support or the direct solidification of a large number of DNA probes on the support in a microprinted manner (called DNA microarray). The surface of the object is then hybridized with the labeled sample, and the genetic information of the sample can be obtained by detecting and analyzing the hybridization signal. Because computer silicon chips are commonly used as solid supports, they are called DNA chips. In addition to DNA, the probes immobilized on the chip can also be cDNA, oligonucleotides or gene fragments from the genome, and these probes are solidified on the chip to form a gene probe array. Therefore, DNA chips are also called gene chips, cDNA chips, oligonucleotide arrays, and biochips. DNA c...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 曾纪晴张明永
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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