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Application of Natural Brassinolide in Capsicum

A technology of brassinolide and capsicum, which is applied to the application field of natural brassinolide on capsicum, can solve the problem of no indication of natural brassinolide analogues, no indication of the effect of anti-low temperature, brassinolide. Lactone waste and other problems, to achieve the effect of diluting the cost, stabilizing the process, and promoting the growth of pepper

Active Publication Date: 2019-03-19
CHENGDU NEWSUN CROPSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the Chinese patent application CN1269140A describes the anti-plant disease composition comprising brassinolide, it does not suggest its independent anti-low temperature effect, nor does it suggest the natural brassinolide analogue of the present invention; although China Patent application CN1930966A describes a fungicidal composition containing brassinolide, but there is no indication of its independent anti-low temperature effect, nor does it suggest the natural brassinolide analogs of the present invention; in addition, although Chinese patent application CN102617236A describes the The fungicidal composition of brassinolide, but there is no suggestion of its anti-low temperature effect alone, nor the natural brassinolide analogue of the present invention, and more seriously, the brassinolide used therein is relatively The content of phosphorus and other elements is extremely high, resulting in the waste and reduction of brassinolide

Method used

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  • Application of Natural Brassinolide in Capsicum
  • Application of Natural Brassinolide in Capsicum
  • Application of Natural Brassinolide in Capsicum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Extraction and Identification of Natural Brassinolide Analogs

[0051] Take 100 grams of rapeseed pollen, add 300 mL of 95% (V / V) ethanol, ultrasonically crush, filter, and retain the filtrate; add 300 mL of 95% (V / V) ethanol to the filter residue, ultrasonically crush, filter, and retain the filtrate. Combine the filtrates, dry and concentrate at 75°C under a vacuum of 0.085Mpa to a volume of 100mL to obtain pollen alcohol extract.

[0052] Add 200 mL of 50% (V / V) ethanol to the pollen alcohol extract, mix well, then add 500 mL of ethyl acetate for extraction, keep the upper layer (ethyl acetate layer); continue adding 500 mL of ethyl acetate to the lower layer for extraction, keep the upper layer ( ethyl acetate layer). Combine the ethyl acetate layers, add 300mL enzyme solution (that is, the mixed enzyme solution of lipase and glucoamylase, wherein lipase (purchased from Jining Hemei Bioengineering Co., Ltd.) 2500U / L, glucoamylase (purchased from Jining He...

Embodiment 2

[0057] Example 2 Determination of natural brassinolide analogs endowing plants with anti-low temperature stress activity

[0058] Through low temperature stress experiments, we measured the plant height, leaf number, leaf area, tiller number, root number and other parameters of rice seedlings after low temperature stress to fully reflect the effect of natural brassinolide analogues on plant low temperature resistance.

[0059] Take the natural brassinolide analogs BR1, BR2, BR3, BR4, BR5 and BR6 prepared in Example 1, respectively, and dissolve them in 95% (V / V) ethanol, and then use a large amount of pure water to gradiently dilute to 1 mg / L, 0.1mg / L, 0.01mg / L, 0.001mg / L and 0.0001mg / L; at the same time, pure water was used as a control.

[0060] After the rice seeds were sterilized with 5% sodium hypochlorite solution, rinsed with distilled water until alkaline, placed in a petri dish, and placed in a thermostat at 30°C ± 2°C for 9 days in dark hydroponics. Then, take out ...

Embodiment 3

[0086] The capsicum cultivation experiment of embodiment 3 natural brassinolide analogs

[0087] According to the enlightenment of the experimental results in Example 2, we comprehensively reflect the effect of natural brassinolide analogues on pepper cultivation according to the yield and maturity period by cultivating peppers.

[0088] BR1, BR2, BR3, BR4, BR5 and BR6 were mixed into a mixture of brassinolide analogs (abbreviated as "BR mix"; wherein the weight ratio of BR1: BR2: BR3: BR4: BR5 and BR6 was 0.4: 0.4: 0.4: 0.4:0.4:98); at the same time, pure water was used as a negative control. Mix and dilute BR to the following concentrations: 0.1ppm, 0.01ppm, 0.001ppm, 0.0001ppm, and cultivate the early-maturing pepper variety "Ganfeng No. 5" in the experimental field, where the cultivation conditions (temperature control, fertilizer application, etc.) refer to Jiangxi Academy of Agricultural Sciences' instructions for the "Ganfeng No. 5" sold. In short, this test was sown ...

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Abstract

The invention relates to the application of natural brassinolide to hot pepper, and particularly provides a method for cultivating hot pepper. The method comprises the step of applying brassinolide or brassinolide analogue in the nursery stage and after setting date of hot pepper, preferably, applying the natural brassinolide analogue with stable ratio. In addition, the invention further provides the application of the natural brassinolide analogue.

Description

technical field [0001] The present invention is a continuation application of Chinese Patent No. 201210026285.7, and belongs to the field of agrochemical technology. Specifically, the present invention relates to a method for cultivating peppers, wherein brassinolide or its analogs are applied at the seedling raising stage and after planting, especially Administration of natural brassinolide analogs of the invention. Background technique [0002] Brassinolide is internationally recognized as the most active, broad-spectrum, and non-toxic plant growth regulator. Its physiological benefits are higher than the other five types of plant hormones that have been discovered, and it is known as the sixth largest type of plant hormones in the world. . Brassinolides are widely found in angiosperms, gymnosperms and some lower plants. From the perspective of distribution in plants, they exist in roots, stems, leaves, pollen, pistils, fruits and seeds, such as rapeseed pollen Contains ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/05C07J73/00C07J9/00C07J75/00A01N45/00A01N43/22A01P21/00
Inventor 何其明
Owner CHENGDU NEWSUN CROPSCI
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