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Method for culturing adventitious roots of oplopanax elatus Nakai by utilizing bioreactor

A bioreactor, thorny ginseng technology, applied in the biological field, can solve the problems of residual harmful substances, land competition between medicinal materials and crops, unstable content of effective substances, etc., achieve low shear force, solve the difficulty of artificial cultivation, and solve the problem of fluidity. uniform effect

Inactive Publication Date: 2013-06-12
YANBIAN UNIV
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Problems solved by technology

However, in field cultivation, plant growth is limited by climate, geography and seasons, and due to the use of pesticides and fertilizers in cultivation management, harmful substances remain; at the same time, the content of effective substances is unstable, and the competition between medicinal materials and crops is also a problem that plagues Chinese traditional medicine. Difficulties in the development of the material industry

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  • Method for culturing adventitious roots of oplopanax elatus Nakai by utilizing bioreactor

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Embodiment Construction

[0014] Such as figure 1 , using an air-lift bioreactor to cultivate the adventitious roots of Northeast thorns, figure 1 Middle a: plug; b: air pump; c: air flow meter; d: filter membrane; e: porous nozzle (pore size 15 μm); f: air outlet; the arrow indicates the direction of air flow.

[0015] Concrete implementation steps are as follows:

[0016] (1) Materials and methods

[0017] 1. Bioreactor Culture of Adventitious Roots of Northeast Ginseng

[0018] ①. Screening of IBA concentration

[0019] Into a 5 L air-lift bioreactor, inject 4 L of liquid medium, the medium is MS (the medium commonly used in plant tissue culture) and add different concentrations of IBA (indole acetic acid) (2, 3, 4, 5 and 6 mg / L), pH adjusted to 5.8, at a temperature of 121°C, a pressure of 1.2 kg / cm 2 Under the conditions of high pressure sterilization for 40 min, after cooling, inject into the reactor, then insert 20 g adventitious roots into each reactor, inject 50 mL / min (air volume / cultu...

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Abstract

The invention discloses a method for culturing adventitious roots of oplopanax elatus Nakai by utilizing a bioreactor. The method comprises the steps of: injecting 4L of an MS culture medium in the 5L airlift bioreactor and adding 3-4mg / L of IBA (Iso Butyl Alcohol) and 40-50g / L of white sugar, wherein the pH is 5.8; injecting air at 75-100mL / min; and inoculating 20-40g of adventitious root explants, and culturing for 40-50 days in dark, so as to obtain a large number of adventitious roots of the oplopanax elatus Nakai. The total phenols content in each obtained adventitious root of the oplopanax elatus Nakai is higher than that in the root and the stem of cultivated oplopanax elatus Nakai with two or five growth years and the polysaccharide content in the adventitious roots is approximate to that in the root of the cultivated oplopanax elatus Nakai with the two growth years. The method is capable of accelerating the multiplication rate, so as to obtain a large number of explants within a short period. The bioreactor is used for cultivation, the cost of the culture medium can be reduced, the labor intensity and the production cost can be lowered, the problems that currently the wild oplopanax elatus Nakai plants are endangered and the artificial cultivation difficulty o the oplopanax elatus Nakai plants is high are solved and a new approach is provided for the commercialized production.

Description

Technical field [0001] The present invention involves the field of biotechnology, and specifically involves a method that uses biological reactors to cultivate northeast assassin. Background technique [0002] Northeast Assassin ( Oplopanax ellaatus NAKAI), also known as thorns, has the genus of Wujiake, and has perennial deciduous shrubs. It is a medicinal plant unique to Changbai Mountain in my country.The distribution area of Northeast Assassin is very narrow, mainly distributed in the Governor of Jilin Governor, and Russia and North Korea are also distributed.In the past 10 years, natural resources have been severely damaged due to a large number of excavations.At present, the natural Northeast Assassin's ginseng resources in Changbai Mountain have been endangered and are listed as a national second -category protected plant. [0003] The root and stems of the Northeast Assassin containing a variety of saponin, flavonoids, volatile oils, pyrine, fatty acids, proteins, sugars,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 廉美兰朴炫春高日于丹吴春花吴松权
Owner YANBIAN UNIV
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