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Method for constructing network regulation and control of chicken muscle creatinine synthetic path related key enzyme genes

A technology of key enzyme genes and synthetic pathways, applied in special data processing applications, instruments, electronic digital data processing, etc., can solve the problem of low reliability of results

Active Publication Date: 2013-06-12
YANGZHOU UNIV
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Problems solved by technology

This method needs to assume that the data satisfies a normal distribution, and this assumption has not been confirmed, resulting in low credibility of the results

Method used

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  • Method for constructing network regulation and control of chicken muscle creatinine synthetic path related key enzyme genes
  • Method for constructing network regulation and control of chicken muscle creatinine synthetic path related key enzyme genes
  • Method for constructing network regulation and control of chicken muscle creatinine synthetic path related key enzyme genes

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Experimental program
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Effect test

Embodiment Construction

[0037] 1 Materials and methods

[0038] 1.1 Materials

[0039] 1.1. Test animals

[0040] The establishment of experimental chicken flocks: all-sibling Rugao yellow chicken family, from the national and local poultry resource gene bank of the Institute of Poultry, Chinese Academy of Agricultural Sciences, muscle tissues were collected at 2, 4, 6, 8, 10, and 12 weeks of age The samples were about 2g, and each age was 6 (3♂, 3♀), quick-frozen in liquid nitrogen, and stored at -70°C for later use.

[0041] 1.2 Gene chip experiment

[0042] The proposed muscle RNA was tested using Agilent expression profile chip technology, and Quant Array 3.0 software was used to analyze the sample point signal-to-noise ratio (signal noise ratio, SNR). Statistically analyze the average SNR (mSNR) of each key enzyme gene and blank control point after hybridization with a data analysis program to determine the strength of the hybridization signal. At the same time, data filtering was performed ...

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Abstract

The invention belongs to the field of animal breeding science, biology of development and cytobiology, and relates to a method for analyzing and controlling network regulation and control by using expression pattern data and a related biological information tool. The method comprises the steps of: firstly, establishing a data set of creatinine related genes, calculating a weight model of each gene, preliminarily selecting gene expression regulation and control; secondly, solving related coefficients and solving, and constructing a network diagram; and finally, by combining with the established integer nonlinear planning model, and on the basis of a KEGG (Kyoto Encyclopedia of Genes and Genomes) database, constructing a network regulation and control diagram of the creatinine related genes. According to the invention, Rugao yellow chickens as a local chicken variety in China are used as materials to construct oligonucleotide probes of eight key enzymes in an IMP synthetic path, an RNA (Ribose Nucleic Acid) level-based regulation and control model is screened on the basis of molecular hybridization, and expression difference of a favor material, i.e. IMP synthetic key enzyme, on the transcriptional level is disclosed. The method provides a theoretical foundation for cultivation and industrial development of excellent-quality chickens.

Description

technical field [0001] The invention belongs to the fields of animal breeding, developmental biology and cell biology. It relates to a method for analyzing and constructing network regulation by using expression profile data and related biological information tools. Background technique [0002] Involved in the molecular regulation mechanism of inosinic acid (IMP) synthesis pathway, generally speaking, in the whole IMP synthesis pathway, especially in the process of forming imidazole five-membered ring in the first stage and pyrimidine six-membered ring in the second stage, The regulation of key enzymes is the main cause of the difference in expression level. However, there are few studies on the key enzymes of the IMP synthesis pathway. Through the screening of candidate genes SNP (single nucleotide polymorphism) related to inosinic acid content in local chicken breeds, it was found that the adenosylsuccinate lyase gene 1 (ADSL) locus can be used as Secondary selection ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/12
Inventor 陈国宏常国斌栾德琴陈蓉张颖马腾夏明秀戴爱琴翟飞刘璐陈静王洪志徐璐
Owner YANGZHOU UNIV
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