Method for detecting L-cysteine concentration by applying of circular dichroism

Abstract

The invention discloses a method for detecting L-cysteine concentration by applying of a circular dichrorism, and belongs to the technical field of analytical chemistry. The L-cysteine and a reagent are mixed according to a certain volume ratio to generate self-assembly and form gold nanoparticle dimer, reactant is placed on a circular dichroism analyzer, signal strength at 525nm position of a main wave is detected, and therefore the concentration of the L-cysteine is measured. Compared with a traditional instrument, under a liquid environment, AuNPs and the L-cysteine are utilized to react to form the gold nanoparticle dimer, the chirality of the L-cysteine can be enlarged through the form of a binary assembly, and a CD signal is produced by the gold nanoparticle diamer in a visible region. The contents of the L-cysteine are measured by a manner that the CD signal intensity under the liquid environment is measured through the circular dichrorism. The method only can be carried out under the liquid environment, and a step of washing is not needed. Only one step of reaction is needed, reaction conditions are simplified, and sensitivity of detection is improved.

Description

technical field [0001] A method for detecting the concentration of L-cysteine ​​by using circular dichroic spectroscopy, which relates to a method for measuring the concentration of L-cysteine ​​by combining gold nanoparticle self-assembly technology and plasma chiral technology, which belongs to analytical chemistry technology field. Background technique [0002] Currently commonly used cysteine ​​detection methods are based on physical or chemical methods, such as inductively coupled plasma emission spectrometry, flow injection atomic absorption spectrophotometer, inductively coupled plasma mass spectrometry, polarography and voltammetric electrodes, Atomic fluorescence spectroscopy, atomic furnace absorption spectroscopy. The biggest advantage of the above-mentioned main instrument detection methods is that they are accurate, but these traditional methods require complicated pre-treatment, long analysis time, high labor intensity and high cost, and can only be performed ...

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