Shewanella piezotolerans 34# and application thereof to algae inhibition

A Shewanella, pressure-resistant technology, applied in marine pressure-resistant Shewanella bacteria and its application in inhibiting the growth of red tide algae, can solve the lack of pertinence of strains, and the lack of strict distinction between red tide occurrence period and non-occurrence period And other issues

Inactive Publication Date: 2013-06-26
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In previous studies, the screening of algicidal bacteria did not strictly distinguish between the red tide occurrence period and the non-occurrence period, making the screened strains lack of pertinence; in addition, the research on whether the algicidal bacteria have the property of secreting quorum sensing signals has not been involved

Method used

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  • Shewanella piezotolerans 34# and application thereof to algae inhibition
  • Shewanella piezotolerans 34# and application thereof to algae inhibition
  • Shewanella piezotolerans 34# and application thereof to algae inhibition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, screening, identification and characteristics of pressure resistant bacterium (Shewanella piezotolerans) 34#

[0046] 1. Screening of Shewanella piezotolerans 34#

[0047] On April 21, 2011, a localized red tide occurred near the coast of the South China Sea (Shenzhen Shekou Port) (the surface layer of the red tide occurrence area of ​​Shenzhen Shekou Port). Phylum Bacillariophyceae, Fragilariaceae Schroder, genus Thalassionema Grunow. Retrieve the water sample from this area, bring the water sample back to the laboratory and filter it through gauze to remove impurities and large particles in the water; then filter it through a 100μm metal mesh to remove debris in the water; finally filter it through a 10μm filter membrane to remove algae cells , and the filtrate was coated and screened for bacteria.

[0048] Dilute the filtrate prepared above, take 30 μL and spread it on the 2216E seawater solid medium (recipe: peptone 5.0g, yeast extract 1.0g, iron pho...

Embodiment 2

[0062] Embodiment 2: the detection of the QS ability of Shewanella piezotolerans (Shewanella piezotolerans) 34# production

[0063] 1. Preparation of Shewanella. The 34# bacterial strain of identification is cultivated in the eppendof tube of 1.5 milliliters for 6-8 hours, and the liquid volume of the eppendof tube is 0.5 milliliters, and the substratum is LB liquid medium (medium formula: tryptone 10g, yeast extract 5g, NaCl 10g, adjust pH to 7.0. Dilute to 1L with deionized water. Steam sterilize at 15psi high pressure for 20min). The concentration of the cultured Shewanella liquid was detected by a visible light spectrophotometer, and the concentration value was about OD 600 = 0.35. The prepared bacterial solution was stored in a 4°C refrigerator for later use.

[0064] 2. Preparation and detection of test strains. The QS produced by the 34# strain was detected with the reporter strains CV026 and A136 that specifically detect QS, wherein the reporter strain CV026 is sui...

Embodiment 3

[0066] Embodiment 3, the application of Shewanella piezotolerans (Shewanella piezotolerans) 34# in inhibiting algae

[0067] 1. The method of inhibiting algae by Shewanella piezotolerans 34#

[0068] 1. High-density culture of Shewanella piezotolerans 34#

[0069] Carry out primary culture (2216E liquid culture medium, formula: peptone 5.0g, yeast extract 1.0g, ferric phosphate 0.01g, old seawater 1000mL, adjusted by the pressure-resistant Shewanella 34# obtained by embodiment 1 in a 10mL conical flask. ph to 7.8), the culture condition is 30°C, the rotation speed is 200r / min, and the time is 6h, when the strain density is close to OD 600 = 0.5, transfer to a 100mL Erlenmeyer flask for secondary culture. Continue to cultivate for 18 hours until the strain density reaches 1×10 9 Individual / ML concentration, spare.

[0070] 2. Cultivation of algae cells for experiments

[0071] The algal species used in the experiment was Alexandrium catenella (Hong Kong strain). Take the ...

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Abstract

The invention discloses shewanella piezotolerans 34# and an application thereof to algae inhibition. The collection number of the shewanella piezotolerans 34# is CGMCC NO. 6490, and the shewanella piezotolerans 34# has the capacity of producing a long-chain QS (quorum sensing) signal and the function of inhibiting alga. A method provided by the invention has a good effect of inhibiting the red-tide algae, can be used for preventing red-tide organisms effectively, can realize the biological antagonistic prevention and control concept of controlling alga by bacteria and has field popularization value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a marine pressure-resistant Shewanella bacterium and its application in inhibiting the growth of red tide algae. Background technique [0002] Red tide refers to the ecological phenomenon (mostly red or brown) in which plankton (especially algae) explodes in a short period of time and causes abnormal water color. It is called "algal bloom" or "algal bloom". The Red Ghost". The increasing pollution of the offshore environment and the unreasonable development of marine resources have brought huge pressure on the ecological environment, resulting in frequent occurrence of local red tides and ecological disasters in the marine environment. This ecological disaster has caused water pollution, energy consumption, and the reduction of dissolved oxygen in water, posing a serious threat to the living environment of organisms. Some red tide organisms also produce toxins, which directly kill ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C02F3/34C12R1/01
Inventor 周进银鹏
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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