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A Scanning Sampling and Image Processing Method for Rapid Imaging

An image processing and image processing device technology, applied in the field of optical scanning, can solve problems such as weak image signal intensity, image detail distortion, and short exposure time, and achieve the effects of fast readout speed, low distortion, and high signal-to-noise ratio

Inactive Publication Date: 2017-02-08
HUAZHONG UNIV OF SCI & TECH
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  • Application Information

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Problems solved by technology

However, due to the fast scanning imaging speed and short exposure time, under the same optical power, only the subarray (or ROI) working mode of the sCMOS camera is used for sampling imaging, and the obtained image of the entire sample has weak signal intensity and low contrast
The larger the N value of the number of pixel rows included in the sampling frame at the same time, the lower the sampling resolution, resulting in more serious image detail distortion.

Method used

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  • A Scanning Sampling and Image Processing Method for Rapid Imaging
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  • A Scanning Sampling and Image Processing Method for Rapid Imaging

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Embodiment Construction

[0024] In this specific embodiment, for the high-speed imaging working mode of the new detection device sCMOS Flash2.8, a scanning sampling and image processing method based on image shifting and superposition is first proposed to solve the problem of rapid continuous scanning and sampling of imaging objects based on platform movement. The problem of weak signal strength and low resolution in imaging.

[0025] Such as figure 1 As shown in the schematic diagram of the scanning sampling system, the imaging object is placed on the translation stage. During the scanning and sampling process, the illumination beam and the detector sCMOS do not move, but the translation stage moves. The speed of movement is related to the frame rate and sampling method of the detector. In the figure, 1 is an sCMOS camera, 2 is a lens, 4 is a half mirror, 5 is an objective lens, 6 is a platform where the sample is placed, and 7 is a light source. Place the imaging object on the moving platform, and the...

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Abstract

The invention discloses a scanning sampling and image processing method for rapid imaging. It uses an sCMOS camera as an imaging tool and makes the sCMOS camera work in subarray (or ROI) mode, including the following steps: using a moving platform to continuously translate the imaging object at a set speed in the direction perpendicular to the sCMOS camera sampling direction, and using the sCMOS camera to The object is scanned and imaged, and the sampling frame is output. Finally, each obtained image frame is shifted and superimposed to obtain an imaging map. This invention takes advantage of the high-speed sampling characteristics of the sCMOS camera in the subarray (or ROI) mode, and performs shift and superposition processing on the sampling frames of the sCMOS camera. The generated image has the advantages of high resolution, high signal-to-noise ratio and low distortion, especially Suitable for fast imaging of large-sized objects.

Description

Technical field [0001] The present invention relates to the field of optical scanning technology, in particular to a scanning sampling and image processing method using scientific research-grade CMOS, that is, sCMOS for fast imaging. Background technique [0002] With the development of modern industry and technology, the demand for rapid imaging has become stronger, especially for imaging large-volume or large-area objects. For example, in biological research, understanding the structure of biological tissues and organs has a great promotion effect on mastering their functions, and provides a strong scientific basis for the diagnosis of various functional diseases. However, due to the large size of tissues and organs (such as the brain), the imaging range is limited, and multiple regional imaging is required to obtain the complete tissue structure data of a plane; at the same time, because of the specificity of the organism, it is necessary to conduct imaging studies on a large ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H04N5/225H04N5/232H04N5/243H04N23/76
Inventor 骆清铭曾绍群龚辉郑廷杨涛
Owner HUAZHONG UNIV OF SCI & TECH