Ophthalmic preparation containing lysozyme

An ophthalmic preparation and lysozyme technology, which is applied in the fields of enzymology and pharmaceutical preparation, can solve the problems of inability to store for a long time, limitation of lysozyme, poor stability of lysozyme, etc.

Active Publication Date: 2013-07-03
SHENYANG XINGQI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, lysozyme has poor stability in water and cannot be stored for a long time
And most ophthalmic preparations a

Method used

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  • Ophthalmic preparation containing lysozyme
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  • Ophthalmic preparation containing lysozyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: the preparation of lysozyme preparation

[0056] (1) Get 0.5g of lysozyme raw material and add in 100g of water for injection, stir and dissolve, and wait for all to dissolve and set aside;

[0057] (2) Add 16 g of sodium dihydrogen phosphate thereto, and use 1mol / L sodium hydroxide to adjust the pH value of the water for injection to 5.0 ± 0.2;

[0058] (3) Stand still at 20°C for 48 hours, then filter with a mixed cellulose membrane;

[0059] (4) Add 0.02 g of activated carbon, adsorb at 70°C for 20 minutes, filter the activated carbon through a Buchner funnel, and filter the obtained solution.

[0060] (5) Put the processed solution into a low-temperature refrigerator and pre-freeze at -70°C. Usually, after 8 hours of pre-freezing, take it out and put it into a cooled cold well. The initial temperature of the cold well is between -40°C. After vacuuming for 1 hour, slowly raise the temperature to -20°C, continue vacuuming for 10 hours, and transfer to...

Embodiment 2

[0061] Embodiment 2: the preparation of lysozyme preparation

[0062] (1) Get 6.2g of boric acid, add 0.029g of borax in 100g of water for injection, stir and dissolve, and wait for all to dissolve for later use;

[0063] (2) Add 2.5g lysozyme raw material to it, make its pH value to 6.3±0.3;

[0064] (3) Refrigerate and stand at 4°C for 2 hours, then filter through a vertical melting funnel;

[0065] (4) Add 2 g of bentonite, absorb at 80° C. for 5 minutes, filter through a sand filter to remove the bentonite, and obtain the treated lysozyme preparation for future use.

Embodiment 3

[0066] Embodiment 3: the preparation of lysozyme preparation

[0067] (1) Get 4.0 g of sodium sulfate and add it to 100 g of water for injection, stir and dissolve, and wait for all to dissolve for later use;

[0068] (2) 5.0g lysozyme raw material is added thereto, and sodium hydroxide is used to adjust the pH value to 8.0±0.2;

[0069] (3) Stand at room temperature (25°C) for 6 hours, and filter with a sand filter;

[0070] (4) Add 10.0 g of activated carbon, absorb at room temperature (25°C) for 24 hours, remove the activated carbon by filtering through a sand filter, adjust the pH value to 10.8±0.3 with 10% concentrated sodium hydroxide solution, settle the lysozyme solid at isoelectric point, and collect the solid by filtration , At 30°C, vacuum-dry for 24 hours to obtain the lysozyme preparation.

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Abstract

The invention belongs to the field of enzymology and medicinal preparations, and relates to an ophthalmic preparation containing lysozyme. Specifically, the content of the lysozyme in the ophthalmic preparation is 0.001%-25% (w/w), 0.01%-20% (w/w), 0.1%-15% (w/w), 0.3%-10% (w/w) or 0.5%-5% (w/w). The ophthalmic preparation and the lysozyme therein have good stability and good performances of bacteriostasis and/or antisepsis and are helpful for long-term storage.

Description

technical field [0001] The invention belongs to the fields of enzymology and pharmaceutical preparation, and relates to an ophthalmic preparation containing lysozyme. Background technique [0002] In recent years, a large number of clinical data and research materials of ophthalmic pharmaceutical preparations have shown that side effects or adverse reactions during the treatment of ocular diseases are often caused by bacteriostatic agents. For example, when drugs are used for postoperative treatment after eye surgery, the phenomenon of corneal epithelial cell shedding due to the influence of bacteriostatic agents often occurs. Moreover, with the popularity of computers and air conditioners, the number of patients with dry eye syndrome is also increasing. The treatment of such patients often requires long-term supplementation with artificial tears, and the long-term use of chemical antibacterial agents will cause varying degrees of corneal damage. [0003] Therefore, the sid...

Claims

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Application Information

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IPC IPC(8): A61K38/47A61P27/02
Inventor 刘继东唐海王征月
Owner SHENYANG XINGQI PHARM CO LTD
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