Device for detecting flour brightening agent and application method thereof
A whitening agent and flour technology, applied in laboratory containers, chemical instruments and methods, material excitation analysis, etc., can solve problems such as the limitations of the industrialization of microfluidic chips, achieve simplified driving devices, and fast detection speed , the effect of simple structure
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Embodiment 1
[0034] see Figure 1 to Figure 3 , the device structure of the present invention is as follows:
[0035] It is in the shape of an optical disc and includes a base A and a cover B. The base A and the cover B are designed to be sealed and bonded to each other. Wherein, six microfluidic channel units are evenly arranged on the substrate A along the circumferential direction, and each microfluidic channel unit is distributed along the radial direction.
[0036] See especially image 3 , each microfluidic channel unit includes: a liquid storage pool 2, a sample chamber 4, a filter area 5 and a detection area 6, which are connected in series and distributed from the center of the substrate A to the edge direction, wherein the liquid storage A breakthrough valve 3 is also provided between the pool 2 and the sample chamber 3 .
[0037] On the cover sheet B, a liquid inlet 1 is provided at a position corresponding to each liquid reservoir 2, and a detection port 6' is provided at a ...
Embodiment 2
[0045] Design and manufacture a centrifugal microfluidic chip for the detection of flour whitening agents, using quartz glass as the chip substrate, using conventional photolithography and wet etching methods to process the channel structure; using polydimethylsiloxane (PDMS) as cover material. In order to increase the capacity of the liquid reservoir and the sample chamber, a double-sided structure of the base and the cover is adopted, and a larger cavity is generated for liquid and sample storage through alignment and bonding. The flour samples spiked with benzoyl peroxide 10PPM, 100PPM, and 1000PPM were pre-loaded into the sample chamber, aligned and bonded to the cover slip. Place the bonded centrifugal microfluidic chip on the centrifugal drive device, clamp the slot and tighten the rotor. Use a micro-syringe to inject the organic solvent acetone into the liquid inlet, and the liquid enters the liquid reservoir. Start the centrifuge and centrifuge at 400rpm for 3 minute...
Embodiment 3
[0047] Design and fabricate a centrifugal microfluidic chip for the detection of flour whitening agents, using PMMA as the chip substrate, using a laser marking machine to process the channel structure; using polydimethylsiloxane (PDMS) as the cover material. Similar to Example 1, the double-sided structure of the substrate and the cover sheet is adopted, and through alignment and bonding, a larger cavity is generated for liquid and sample storage. The flour samples spiked with benzoyl peroxide 10PPM, 100PPM, and 1000PPM were pre-loaded into the sample chamber, aligned and bonded to the cover slip. Place the bonded centrifugal microfluidic chip on the centrifugal drive device, clamp the slot and tighten the rotor. Use a micro-syringe to inject the organic solvent acetone into the liquid inlet, and the liquid enters the liquid reservoir. Start the centrifuge and centrifuge at 600rpm for 3 minutes. The acetone in the reservoir enters the sample chamber through the breakthrough ...
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