Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of using lipase to catalyze and synthesize galactose-6-acetate on line

A technology of galactose and lipase, which is applied in the field of lipase-catalyzed online controllable selective synthesis of galactose-6-acetate, can solve the problems of low conversion rate and selectivity, long reaction time, etc., and shorten the reaction Effect of time, reduced usage, high conversion and reaction selectivity

Active Publication Date: 2013-07-03
ZHEJIANG UNIV OF TECH
View PDF2 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, many domestic and foreign scholars have studied the enzyme-catalyzed synthesis of mannose esters in organic media, but this method often requires a long reaction time (24h), and the conversion rate and selectivity of the reaction are not high, so We studied the lipase-catalyzed selective synthesis of galactose-6-acetate in a microchannel reactor, aiming to find an efficient and environmentally friendly online controllable selective synthesis method of galactose-6-acetate monoester

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of using lipase to catalyze and synthesize galactose-6-acetate on line
  • Method of using lipase to catalyze and synthesize galactose-6-acetate on line
  • Method of using lipase to catalyze and synthesize galactose-6-acetate on line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the synthesis of galactose-6-acetate

[0027]

[0028] device reference figure 1 : Dissolve galactose (0.4mmol) in 10mL of tert-amyl alcohol: DMSO=4:1 (v / v) mixed solvent, vinyl acetate (8.8mmol) is dissolved in 10mL of tert-amyl alcohol, and then respectively packed in 10mL Ready to use in the syringe. 0.87g of lipase Lipozyme TLIM was evenly filled in the reaction channel of the microfluidic channel reactor, and driven by the PHD2000 syringe pump, the two reaction solutions were separated at 10.4 μL min -1 The flow rate enters the reaction channel through the "Y" joint for reaction, and the temperature of the reactor is controlled at 52 ° C by a water bath thermostat. The reaction solution flows continuously in the reaction channel for 30 minutes, and the reaction results are tracked and detected by thin-layer chromatography (TLC).

[0029] The reaction solution was collected online by the product collector, the solvent was distilled off under reduc...

Embodiment 2-5

[0033] Change the temperature of the microfluidic channel reactor, others are the same as in Example 1, and the reaction results are as shown in Table 1:

[0034] Table 1: Effect of Temperature on Reaction

[0035]

[0036] The results in Table 1 show that when the flow rate is 10.4 μL min -1 , when the reaction time is 30min, the conversion rate increases obviously with the increase of the reaction temperature. When the reaction temperature reaches 52°C, the conversion rate and selectivity of the reaction are the best. The decrease in enzyme activity leads to a decrease in the conversion rate and selectivity of the reaction, so the optimal reaction temperature of galactose acetate in the microfluidic microchannel reactor in the present invention is 52°C.

Embodiment 6-10

[0038] Change the substrate ratio of vinyl acetate and galactose in the microfluidic microchannel reactor to be 18:1 (Example 6), 19:1 (Example 7), 20:1 (Example 8), 21:1 (Example 9), 23:1 (Example 10), others are the same as Example 1, and the results are shown in Table 2.

[0039] Table 2: Effect of Galactose to Vinyl Acetate Substrate Ratio on Reaction

[0040] Example

[0041] 9

[0042] The result of table 2 shows, along with the increase of reactant vinyl acetate, the conversion rate of reaction also thereupon increases, and when substrate ratio is 22: 1, the conversion rate of reaction and selectivity are optimal, and galactose is quantified substantially Complete conversion to galactose-6-acetate. Now if continue to increase the consumption of reactant vinyl acetate, will cause the conversion rate of reaction and selectivity to reduce, thereby, the optimal substrate ratio of this reaction is 22: 1, under this reaction condition, galactose is quan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method of using lipase to catalyze and synthesize galactose-6-acetate on line. The method comprises the following steps: taking galactose and vinyl acetate as raw materials according to molar ratio of 1:18-23, taking 0.5-1.0 g of lipase Lipozyme TLIM as a catalyst, taking tert-amyl alcohol and DMSO (dimethyl sulfoxide) mixed solvent as a reaction solvent, uniformly filling the lipase Lipozyme TLIM into a reaction channel of a microfluidic channel reactor, wherein the inner diameter of the reaction channel of the microfluidic channel reactor is 0.8-2.4 mm, and the reaction channel is 0.5-1.0 m long, continuously introducing the raw materials and the reaction solvent into the reaction channel to perform acylation reaction, controlling the temperature of acylation reaction to be 40-55 DEG C, and keeping the acylation reaction for 15-35 min, on line collecting reaction liquid, and conventionally post-processing the reaction liquid to obtain galactose-6-acetate. The method has the advantages of short reaction time, high selectivity and high yield.

Description

(1) Technical field [0001] The invention relates to a lipase-catalyzed online controllable selective synthesis of galactose-6-acetate. (2) Background technology [0002] Sugar ester is an excellent non-ionic surfactant, which uses natural renewable resource sugar widely existing in nature as raw material, and has broad development prospects. Today's surfactant industry is developing in the direction of green chemistry, which gradually requires non-toxic products, regenerated reaction raw materials, and green reaction processes. As an excellent natural surfactant, sugar esters meet the two requirements of green chemistry: non-toxic products and renewable raw materials, but the reaction process still needs to be further green. [0003] At present, the sugar esters commercially produced at home and abroad are all synthesized by chemical methods. There are many shortcomings in chemical methods, mainly including: the conditions in the reaction process are severe, side reactions ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/02
Inventor 杜理华罗锡平张航诚顾生妹胡玉婷
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com