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Application of isorhapontigenin (ISO) to preparation of drugs with antitumor activity

A technology of iso-emodin and anti-tumor activity, applied in the field of application of iso-emodin in the preparation of anti-tumor active drugs, to achieve broad application prospects, promote tumor cell apoptosis, and improve sensitivity

Inactive Publication Date: 2013-07-10
ZHEJIANG UNIV
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  • Application of isorhapontigenin (ISO) to preparation of drugs with antitumor activity
  • Application of isorhapontigenin (ISO) to preparation of drugs with antitumor activity
  • Application of isorhapontigenin (ISO) to preparation of drugs with antitumor activity

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Embodiment Construction

[0030] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:

[0031] The following implementation does not limit the present invention. Please refer to the corresponding part of the third edition of "Molecular Cloning Experiment Guide" (J. Sambrook, E.F. Fritsch, etc., Science Press) or refer to the reagents used for the operation steps not described in detail in the examples. Instructions for the box. The ISO was provided by Professor Hou Qi from the Institute of Materia Medica, Peking Union Medical College, China. It was dissolved in dimethyl sulfoxide (DMSO) and the solution was prepared and stored with a final concentration of 60mmol / L.

[0032] (1) ISO induces apoptosis by inhibiting the proliferation of bladder cancer T24T cells

[0033] Bladder cancer T24T cells (Huang CS Laboratory, New York University School of Medicine) grew to 70%, starved the cells with 0.1% ...

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Abstract

The invention discloses application of isorhapontigenin (ISO) to preparation of drugs with antitumor activity. The activity of the X-linked inhibitor of apoptosis protein (XIAP) is inhibited by lowering gene expression of the XIAP. Western-blotting, reverse transcription-polymerase chain reaction (RT-PCR), an adenosine triphosphate (ATP) bioluminescence detection method, chromatin immunoprecipitation (CHIP) and other methods are utilized to prove that the XIAP gene expression level can be obviously inhibited, then tumor cell apoptosis is induced and tumor cell proliferation is inhibited by pretreating various tumor cells with ISO, ISO can not obviously induce tumor cell proliferation and apoptosis, the endogenous XIAP gene is the effective target treated with ISO and ISO is used for specifically inhibiting gene expression of the XIAP. Experiments prove that ISO can effectively improve the sensitivity of chemotherapy drugs and promote tumor cell apoptosis and has extensive application prospects in foundation medicine, clinical treatment, new drug research and development and the like.

Description

(1) Technical field [0001] The invention relates to the application of a traditional Chinese medicine monomer iso-emodin (ISO) in the preparation of active antitumor drugs, that is, the application of iso-emodin in inhibiting the activity of X-linked apoptosis inhibitory protein in tumor cells. (2) Background technology [0002] At present, malignant tumors are the main diseases that endanger human health. Among them, the morbidity and mortality of lung cancer, gastric cancer, liver cancer, colorectal cancer and bladder cancer have been increasing significantly. It has the characteristics of early metastasis, late refractory, poor prognosis, and easy drug resistance. However, the curative effects of traditional surgery, chemotherapy, and radiotherapy are not satisfactory. The limitations of traditional treatment methods determine that biological therapy must participate in the comprehensive treatment of malignant tumors, and may play a vital role in it, and gene therapy is o...

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Application Information

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IPC IPC(8): A61K31/085A61P35/00
Inventor 方勇候琦潘宏铭
Owner ZHEJIANG UNIV
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