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Method for analyzing succinic acid fermentation liquid by utilizing near infrared spectrums on line

A near-infrared spectroscopy, succinic acid technology, applied in the field of fermentation engineering, can solve the problem of no succinic acid fermentation liquid, etc., and achieve the effects of good repeatability, simple operation and high accuracy

Inactive Publication Date: 2013-07-10
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no technical scheme for on-line determination of succinic acid fermentation broth at present, in view of this, the present invention is proposed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Microbial culture

[0021] Strain: E.coli BA103 (CCTCC2012032).

[0022] Slant medium: LB medium plus 50 μg / mL each of ampicillin, chloramphenicol, and karithromycin.

[0023] Seed medium: LB medium plus 50 μg / mL each of ampicillin, chloramphenicol, and karithromycin.

[0024] Fermentation medium: glucose 100g / L, yeast extract 10g / L, peptone 10g / L, MnCl 2 0.05g / L, FeCl 2 0.05g / L, CaCl 2 0.05g / L, K 2 HPO 4 2.0g / L, MgCO 3 80g / L, pH7.0. After sterilization, add 50 μg / mL each of ampicillin, chloramphenicol, and karithromycin.

[0025] When cultivating in a 7.5L fermenter, the liquid volume of the medium is 4.5L. After streaking the strains on the seed slant medium, culture them in an incubator. After 36 hours of cultivation at 37°C, the strains were transferred to a 500mL shaker flask containing 50mL seed liquid medium with an inoculation loop at 300rpm at 37°C. After culturing at ℃ for 12 hours, it is used to inoculate the above fermentation medium (7.5L ferm...

Embodiment 2

[0036] (1) Microbial culture

[0037] Strain: Actinobacillus succinogenes NJ113 (CGMCCNo.1716)

[0038] Incline medium: glucose 10g / L, yeast extract 5g / L, peptone 5g / L, NaCl 1g / L, NaHCO 3 10g / L, Na2HPO 4 15g / L, KH 2 PO 4 4g / L, agar 20g / L.

[0039] Seed medium: glucose 10g / L, yeast extract 5g / L, peptone 5g / L, NaCl 1g / L, NaHCO 3 10g / L, Na 2 HPO 4 15g / L, KH 2 PO 4 4g / L, through CO 2 Gas for 2 minutes, sterilized at 121°C for 15 minutes, and cooled.

[0040] Fermentation medium: corn husk hydrolyzate 100g / L, yeast extract 10g / L, peptone 10g / L, MnCl 2 0.05g / L, FeCl 2 0.05g / L, CaCl 2 0.05g / L, K 2 HPO 4 2.0g / L, MgCO 3 80g / L, pH7.0.

[0041]When cultivating in a 7.5L fermenter, the liquid volume of the medium is 4.5L. After streaking the strains on the seed slant medium, culture them in an anaerobic incubator, and control the ratio of mixed gas in the incubator to N 2 :CO 2 :H 2 =8:1:1, the strain after 36 hours of cultivation at 37°C was connected to a 100mL seru...

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Abstract

The invention discloses a method for analyzing succinic acid fermentation liquid by utilizing near infrared spectrums on line. The method comprises the following steps: collecting near infrared spectrums of microorganisms at different culture time on line; measuring dry cell weight, glucose and succinic acid by the conventional method after sampling; preprocessing the near infrared spectrograms and establishing dry cell weight, glucose and succinic acid models respectively by a chemometrics method; and substituting the spectrograms of samples to be tested into the models to acquire fermentation liquid component information of the samples to be tested. The method is quick, high in accuracy, high in repeatability and simple and convenient to operate.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, in particular to a method for online analysis of succinic acid fermentation liquid by using near-infrared spectroscopy. Background technique [0002] Succinic acid is an important C4 platform compound in biorefinery product engineering. It is not only widely used in food, medicine, chemical industry, materials and other fields, but also can be used to synthesize 1,4-butanediol, tetrahydrofuran, γ-butane Esters, bulk chemicals such as N-methylpyrrolidone, and PBS-based biodegradable polyesters. The preparation of succinic acid by microbial fermentation uses renewable biomass resources as raw materials, and can realize the greenhouse gas CO 2 The whole production process has the advantages of green, low energy consumption, high atom economy, less waste, etc., with remarkable social and environmental benefits. At present, in the succinic acid fermentation process, the cell mass in the ferme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
Inventor 陈可泉姜岷王震韦萍欧阳平凯
Owner NANJING UNIV OF TECH
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