Ultrasonic high-energy density bead mill for biological cell wall breaking

Abstract

The invention relates to an ultrasonic high-energy density bead mill used for biological cell wall breaking. The bead mill contains a breaking ultrasonic emission head, a plurality of cleaning ultrasonic emission heads, and several turbines with protruding pins. Vortex grooves of the turbine drive materials and milling beads to make strong radial circulating movement and the protruding pins to generate a strong stirring effect, so that a powerful shearing force can be generated among the milling beads and among the milling beads, the turbine and a cylinder wall so as to break cell walls rapidly. A small number of cell interleaved agglomerates that are still not broken after milling undergo concentrated ultrasonic radiation in a discharge channel, so that the cell agglomerates are dispersed and the walls are broken. In addition, ultrasonic waves can reduce the viscosity of cell fat-containing materials, mitigating adhesion wrapping of cell fat to the milling beads and a milling chamber, and have an excellent cleaning function. Due to the strengthening of the milling strength of the bead mill and the dispersion and breaking functions of auxiliary ultrasonic waves in the invention, the cell wall breaking rate is substantially increased, so that the ultrasonic high-energy density bead mill can be widely applied in the field of biological cell wall breaking.

Description

technical field [0001] The invention belongs to the field of vertical or horizontal bead mills, and in particular relates to an ultrasonic high-energy-density bead mill for breaking biological cells. Background technique [0002] There are three commonly used methods for crushing biological cell walls: one is crushing by bead mill; the other is crushing by ultrasonic technology; the third is crushing by high-pressure technology above 200 kg / cm2 (such as high-pressure fluid collision). Most of the existing cell wall breaking machines are disc or pin pin bead mills. Due to their structure, the fluid circulation in the grinding chamber is poor, dispersed, and the grinding intensity is low, resulting in low wall breaking efficiency and insufficient wall breaking rate. 90%, the grinding time must be extended to increase the wall breaking rate, but when the grinding time is long, the material is easily emulsified, and the useful substances in the cell wall are difficult to separat...

AI Technical Summary

Problems solved by technology

Most of the existing cell wall breaking machines are disc or pin pin bead mills. Due to their structure, the fluid circulation in the grinding chamber is poor, dispersed, and the grinding intensity is low, resulting in low wall breaking efficiency and insufficient wall breaking rate. 90%, the grinding time must be extended to increase the wall breaking rate, but when the grinding time is long, the material is easily emulsified, and the useful substances in the cell wall are difficult to separate in subsequent operations

In addition, the more unfavorable disadvantage is that after the cell wall is broken, the saturated and unsaturated fat in the cell adheres and wraps on the surface of the grinding beads, the grinding chamber cylinder and the stirring rotor. Due to the thick accumulation of fat on the surface, the lubrication of the fat layer and The elastic damping effect will greatly weaken the shearing and squeezing effect between the grinding beads, between the grinding beads and the rotor and the cylinder, so that the wall-breaking efficiency of the bead mill will be greatly reduced, and the machine will work for half an hour to one hour. After that, it must be cleaned once, and the cleaning time is long and the amount of cleaning agent is large, which makes the production efficiency low and the production cost high

The amount of wall-breaking treatment simply using ultrasonic technology is small, and a large amount of processing requires a lot of power. Using high-power crushing, due to uneven energy distribution, areas with insufficient energy, the wall-breaking effect cannot be achieved, or the wall-breaking rate is low; In a large area, the temperature rises too high, causing damage to the cell material, and the overall availability of useful substances for breaking the wall is low

Using high-pressure technology to break the wall, the equipment cost is high, and the use and operation must be very rigorous. A slight negligence or leakage or rupture will cause major safety accidents and endanger the personal safety of the operator. It is difficult for relevant enterprises to promote the use.

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