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Culture medium for producing fibrinolytic active compound through marine stachybotrys longispora

A technology of Botrytis longospora and culture medium, which is applied in the biological field and can solve the problems of restricting activity evaluation and structure-effect research, low content of fibrinolytic active compounds, etc.

Active Publication Date: 2014-11-26
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the content of the fibrinolytic active compound FGFC1 in the original strain is low, which restricts its further in vivo and in vitro activity evaluation and structure-activity research. Therefore, through large-scale fermentation and metabolic regulation research, efficient fermentation production is the primary problem that must be solved. question

Method used

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  • Culture medium for producing fibrinolytic active compound through marine stachybotrys longispora
  • Culture medium for producing fibrinolytic active compound through marine stachybotrys longispora
  • Culture medium for producing fibrinolytic active compound through marine stachybotrys longispora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1, culture medium 1

[0071] 1.1 Preparation of fermentation medium

[0072] Medium 1: Accurately weigh 125g of glucose, 3.3g of sodium nitrate, 0.07g of dipotassium hydrogen phosphate trihydrate, 0.4g of magnesium sulfate heptahydrate, 0.625g of potassium chloride, 0.7g of yeast extract, and 0.003125g of cobalt chloride hexahydrate , 0.01875g of ferrous sulfate heptahydrate, 0.0065g of anhydrous calcium chloride, 13g of L-ornithine hydrochloride, prepared in 1000ml of deionized water (equivalent to 1000g).

[0073] 1.2 Fermentative production of fibrinolytic active compound FGFC1

[0074] Sea botrytis longuscensus FG216 (obtained from Shanghai Ocean University), washed the sea botrytis longosporins FG216 spores from the slope with sterile water, according to the final concentration of about 3 × 10 6 Each / 100ml was inserted into the seed medium, and cultured on a shaker at 25°C and 180r / min for 60h to obtain a fresh first-grade seed solution; according to t...

Embodiment 2

[0078] Embodiment 2, culture medium 2

[0079] 2.1 Preparation of fermentation medium

[0080] Medium 2: Accurately weigh 125g of glucose, 3.3g of sodium nitrate, 0.07g of dipotassium hydrogen phosphate trihydrate, 0.4g of magnesium sulfate heptahydrate, 0.625g of potassium chloride, 0.7g of yeast extract, and 0.003125g of cobalt chloride hexahydrate , 0.01875g of ferrous sulfate heptahydrate, 0.0065g of anhydrous calcium chloride, 15g of L-ornithine hydrochloride, each component was prepared in 1000ml of deionized water.

[0081] 2.2 Fermentation production of fibrinolytic active compound FGFC1

[0082] Same as step 1.2 of Example 1.

[0083] 2.3 Determination of production of fibrinolytic active compound FGFC1

[0084] Same as step 1.3 of Example 1, the measured content of the fibrinolytic active compound FGFC1 was 10320 mg / L.

Embodiment 3

[0085] Embodiment 3, culture medium 3

[0086] 3.1 Preparation of fermentation medium

[0087] Medium 3: Accurately weigh 58g of glucose, 3g of sodium nitrate, 0.1g of dipotassium hydrogen phosphate trihydrate, 0.5g of magnesium sulfate heptahydrate, 0.5g of potassium chloride, 1g of yeast extract, 0.0025g of cobalt chloride hexahydrate, without Calcium chloride 0.0065g, L-ornithine hydrochloride 10g, add deionized water 1000ml, adjust the pH to 5.8.

[0088] 3.2 Fermentative production of fibrinolytic active compound FGFC1

[0089] The fermentation time was 10 days, and other conditions were the same as step 1.2 of Example 1.

[0090] 3.3 Determination of production of fibrinolytic active compound FGFC1

[0091] Same as step 1.3 of Example 1, the measured content of the fibrinolytic active compound FGFC1 was 3944 mg / L.

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Abstract

The invention relates to a novel culture medium for producing a fibrinolytic active compound through marine stachybotrys longispora. The culture medium is suitable for the growth of the marine stachybotrys longisporam, and can greatly raise the output of the fibrinolytic active compounds FGFC1.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to a culture medium for producing fibrinolytic active compounds by Botrytis elongatus marine and a preparation method thereof. Background technique [0002] At present, most natural medicines originate from land, while the ocean is a huge treasure house of biological resources that is rarely developed at present. In recent years, a large number of new bioactive substances have been isolated from marine organisms, especially invertebrates and microorganisms. Statistics show that 656 new compounds were reported in 2003, and 716 new compounds were reported in 2004. Some of them are in Phase I-III clinical trials. Marine microorganisms, including marine actinomycetes and fungi, are important sources of new active substances. Novel compounds found in the ocean have a higher probability of biological activity than terrestrial organisms. For example, in the In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18C12R1/645
Inventor 蔡孟浩王美霞周祥山张元兴
Owner EAST CHINA UNIV OF SCI & TECH