Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular biological method for predicting height of excellent skiers and skaters through detection of osteocalcin content in serum

A molecular biology, athlete technology, applied in biological testing, material testing products, etc., can solve the problems of interference, poor specificity, and inaccurate quantification, and achieve the effect of easy operation and simple method.

Inactive Publication Date: 2013-08-07
HARBIN INST OF PHYSICAL EDUCATION
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemiluminescence immunoassay, enzyme-linked immunosorbent assay, avidin-biotin enzyme immunoassay and other methods have the advantages of simple operation, rapidity, trace amount, no radioactive isotope pollution and marker degradation, etc. The disadvantage is that the specificity is poor, Hemolysis or lipemia interferes with the measurement, and the quantification is not accurate enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0012] Example: Detection of the level of the molecular marker osteocalcin in the serum of elite ice and snow athletes.

[0013] Select 5 outstanding ice and snow athletes, take 5ml of blood from each vein, and put it in the anticoagulant tube for standby (the anticoagulant tube number is 01-05, set as 5 repetitions), and set it as the experimental group; select 5 ordinary people for vein sampling Blood, 5ml per person, was kept in anticoagulant tubes for standby (the anticoagulant tubes were numbered 06-10, set as 5 replicates), and set as the control group.

[0014] ELISA quantitative detection of osteocalcin in serum, the specific method is as follows:

[0015] 1) The above-mentioned osteocalcin protein was made into a 100 μg / ml solution with PBS, added to a 96-well microplate at 100 μl / well, and adsorbed overnight at 4°C;

[0016] 2) The next day, pour off the unadsorbed protein solution in the overnight coated ELISA plate, and wash 3 times with PBS. Add 3% (mass percent...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a molecular biological method for predicting height of excellent skiers and skaters through detection of osteocalcin content in serum. According the invention, enzyme labeled immunosorbent assay is used to detect and compare expression quantities of osteocalcin in bloods of excellent skiers and skaters and of ordinary people, and since osteocalcin acts on osteoblasts to further influence the height of a person, optimum height of excellent skiers and skaters can be indirectly predicted through the expression level of osteocalcin. Detection results of enzyme labeled immunosorbent assay show that the concentration of osteocalcin in the bloods of excellent skiers and skaters is in a range of 1.50 + / - 0.76 nmol / L, higher than the concentration of osteocalcin in the bloods of ordinary people as the concentration of osteocalcin in the bloods of ordinary people is 1.48 + / - 0.58 nmol / L. Thus, it is proved that high osteocalcin content in serum can be used for judgment of optimum height of excellent skiers and skaters. The molecular biological method provided by the invention lays a solid theoretical and technical foundation for establishment of a scientific system of early prediction and evaluation of potential of skiers and skaters, talent selection and talent training, increases the success rate of talent selection and makes a breakthrough to theoretical and technical difficulties in one-shot talent selection.

Description

technical field [0001] The invention relates to a molecular biology method for predicting the height of excellent ice and snow athletes. It is suitable for the early selection of excellent ice and snow athletes at the protein level, and belongs to the interdisciplinary field of sports science and life science. Background technique [0002] The growth of athletes is accompanied by changes in body shape. The research on athletes' body shape, especially height, is a basic and important link in sports science research. The research results of the height characteristics of my country's outstanding ice and snow athletes will help to establish a perfect and systematic scientific research system, and at the same time play a positive role in building a scientific and systematic height model of my country's outstanding ice and snow athletes, and provide a basis for future physical function, Technical tactics, psychology and the formation of a systematic research system lay a certain t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 赵玉华朱志强刘冬崔鹏金红关伟军
Owner HARBIN INST OF PHYSICAL EDUCATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com