TGF-Beta3 (Transforming Growth Factor Beta3) loaded slow-release tissue engineering synovial sheath

A TGF- and tissue engineering technology, applied in the medical field, can solve the problems of easy adhesion, small range of finger movement, and abnormal secretion of synovial fluid, etc., and achieve good prevention and treatment effects

Inactive Publication Date: 2013-08-14
THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flexor tendon of the finger is ruptured, and after the synovial membrane is damaged, the synovial fluid cannot be secreted normally, resulting in a small range of motion of the finger and easy adhesion

Method used

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  • TGF-Beta3 (Transforming Growth Factor Beta3) loaded slow-release tissue engineering synovial sheath
  • TGF-Beta3 (Transforming Growth Factor Beta3) loaded slow-release tissue engineering synovial sheath
  • TGF-Beta3 (Transforming Growth Factor Beta3) loaded slow-release tissue engineering synovial sheath

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Such as figure 1 As shown, a slow-release tissue engineering synovial sheath loaded with TGF-β3, the chitosan tissue engineering scaffold is composed of a dense layer 1 and a porous layer 2, and the dense layer 1 can effectively block the influence and interference of external factors. The thickness of the porous layer 2 is 200-300um, and the porous layer 2 is loose and mesh-shaped, and contains chitosan slow-release microspheres containing TGF-β3 and synoviocytes as a carrier for cell attachment and growth. The artificial synovialization of the chitosan scaffold was realized.

[0027] Prepare as follows:

[0028] (1) Chitosan asymmetric membrane

[0029] a. Formation of dense layer

[0030] Dissolve chitosan in 2% acetic acid aqueous solution to prepare a 1.5% chitosan solution, then centrifuge at 4°C for 5 minutes to remove impurities, and let stand to remove air bubbles before use; pour 1.0ml of the above solution into a 9cm diameter placed in a petri dish at roo...

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Abstract

The invention discloses a TGF-Beta3 (Transforming Growth Factor Beta3) loaded slow-release tissue engineering synovial sheath. The synovial sheath is characterized by consisting of a dense layer (1) and a porous layer (2), wherein the porous layer (2) is mesh-shaped, and chitosan microspheres containing TGF-Beta3 and synovial cells are loaded into the porous layer (2). According to the synovial sheath, the artificial synovialization of chitosan scaffolds is realized. The TGF-Beta3 loaded slow-release tissue engineering synovial sheath has the advantages that the slow release of the TGF-Beta3 can be realized, the cicatrization resisting effect of the TGF-Beta3 is exerted, the TGF-Beta3 with biological activity is released in a slow and sustained manner, and active substances, such as hyaluronic acid and the like, secreted by synovial survival cells can be utilized so as to be expected to play a role in adhesion prevention after tendon injury repair. The synovial sheath can serve as an ideal material for preventing adhesion after tendon injury repair, and has good preventing and treating effects when the synovial sheath is applied to tendon adhesion in orthopedics.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to a slow-release anti-adhesion tissue engineering synovial sheath for medical use. Background technique [0002] Adhesion and healing of finger flexor tendons after injury leads to unsatisfactory recovery of finger function, which is a major problem in the field of hand surgery that needs to be solved urgently. Among the cytokines released during tendon healing, TGF-β (Transforming Growth Factor beta) is considered to be a key factor for tissue fibrosis, scar formation, and tendon adhesion. As an isomer of TGF-β1 in vivo, TGF-β3 can down-regulate the levels of TGF-β1 and TGF-β2, play the role of TGF-β1 antibody, and inhibit the formation of scar. However, the local content of TGF-β3 in the tendon is low in the body, and the concentration of TGF-β3 in the tendon healing environment cannot be maintained, so it cannot play an obvious anti-adhesion effect. [0003] The synovium is the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/20A61L27/38
Inventor 熊雁蒋科王爱民张正治王子明钟菁
Owner THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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