Method for extracting wheat powdery mildew DNA from catching band of spore catcher

A technology of wheat powdery mildew and trap, which is applied in the field of plant diseases, can solve the problems of not being able to meet the requirements of accurate and rapid disease monitoring, and can not use powdery mildew resistance monitoring, etc., to achieve strong practicability, wide application and accurate results Effect
CN103255129AInactive Publication Date: 2013-08-21INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Publication Date
2013-08-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for extracting wheat powdery mildew DNA from the catching band of a spore catcher, and belongs to the plant disease prevalence monitoring field, wherein the method is used for extracting the DNA of powdery mildew conidiospore collected by the spore catcher. The method comprises the following steps: eluting and wall-breaking wheat powdery mildew conidiospores collected on the catching band of the spore catcher, and extracting and purifying DNA of the wall-broken conidiospores to develop the disease outbreak and prevalence monitoring service through utilizing a molecular biological quantitative research technology. The DNA extracted in the invention can be used for accurately and quantitatively detecting the wheat powdery mildew conidiospores collected with the spore catcher, and can also be directly used for the drug resistance monitoring. The method can be popularized in various regions as a disease monitoring technology.
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Description

(1) Technical field

[0001] The "method for extracting wheat powdery mildew DNA from the capture zone of a spore trap" of the present invention can be used to extract the DNA of powdery mildew conidia collected by the spore trap, and belongs to the field of plant diseases. (2) Background technology

[0002] Wheat powdery mildew caused by the obligate parasitic fungus Blumeria graminis f.sp.tritici is one of the important diseases in wheat production in my country. Improving the prediction level of this disease can effectively control the prevalence of the disease and reduce the use of pesticides. Wheat powdery mildew is a typical airborne fungal disease. The number or density of powdery mildew conidia in the air is an important driving variable for the occurrence and prevalence of the disease. There is a very important relationship between the popular speed, popular length and distribution. Wheat powdery mildew can generally re-infect for 5 to 6 generations in spring. The sp...

Claims

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