Equivalent analysis circuit of gene amplification instrument thermal cycling system based on thermoelectric cooler (TEC)

Abstract

The invention discloses an equivalent analysis circuit of a gene amplification instrument thermal cycling system based on a thermoelectric cooler (TEC). Thermal physical parameters of heat q, temperature T, thermal resistance and thermal capacity C in a heat transfer process of the thermal cycling system are replaced by electrical physical parameters of current I, voltage U, resistance R and capacitance c respectively in an equivalent mode. A thermal cycling system pure circuit model based on the TEC is built. Parameter calculation is performed on electrical apparatus elements of the circuit model according to physical parameters supplied by manufacturers, simulation analysis of the equivalent analysis circuit of the thermal cycling system is achieved by combining software of Multisim and the like, and steady state and dynamic performances of the thermal cycling system are studied and evaluated through simulation results. The equivalent analysis circuit of the gene amplification instrument thermal cycling system based on the TEC successfully avoids that modeling and analysis are performed on the heat transfer process of the thermal cycling system through a method by building of a complicated thermodynamic equation, greatly simplifies a thermal performance analysis process of the thermal cycling system, and can provide visual reference for design and optimization of the thermal cycling system.

Description

technical field [0001] The invention relates to a research circuit of a biological and medical detection instrument, in particular to an equivalent analysis circuit of a thermal cycle system of a gene amplification instrument. Background technique [0002] The existing polymerase chain reaction (PCR) technology is a molecular biology technique that rapidly and massively amplifies specific gene fragments through enzymatic reactions in vitro. The reaction principle of PCR is similar to the natural replication process of DNA. Each thermal cycle consists of three basic steps: denaturation (94°C), annealing (55°C) and extension (72°C). The number of genes doubles after each thermal cycle , the target gene can be amplified a million times after dozens of thermal cycles. In order to ensure the specificity of gene amplification results, the reaction temperature of each cycle step must be strictly controlled, and the thermal cycle system is the core component of the temperature cont...

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Problems solved by technology

[0004] At present, researchers at home and abroad are mostly based on the principle of heat transfer, by establishing the thermodynamic equations of the cold and hot ends of the semiconductor thermoelectric cooler (TEC), using analytical methods and numerical calculation methods to theoretically study the thermal performance of the TEC-based thermal cycle system , this research method is not only very complicated in the solution process, but also inaccurate in the solution results due to the inability to establish the thermal model correctly due to the irregular thermophysical parameters of different parts of the thermal cycle system

In addition, the method of establishing thermodynamic equations is not self-adaptive. If any parameter of the thermal cycle system is changed, the tedious process of solving the equations will be performed again, which will greatly increase the workload of thermal cycle system design and optimization.

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