Bioluminescence tomography reconstruction method

A tomography and bioluminescence technology, applied in computer tomography, diagnosis, echo tomography, etc., can solve the problems of ill-posed reverse reconstruction of bioluminescence tomography, easy generation of smooth solutions, and large distribution range, and achieve accurate Efficient reconstruction, improved reconstruction efficiency, good sparsity effect

Active Publication Date: 2013-09-04
GUANGZHOU BLT INSTR & METER
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Problems solved by technology

However, because BLT is an early imaging, at the cellular and molecular level, the light source signal is weak, and is affected by the scattering and absorption of biological tissues, the signal received by the CCD camera on the body surface will have attenuation and noise, coupled with bioluminescence tomography The inverse reconstruction problem of is a severely ill-posed problem, which makes developing stable and efficient reconstruction algorithms a challenging problem in bioluminescent tomography
but traditional/ 2 The norm regularization method is easy to produce a smooth solution, the energy distribution of the reconstructed light source is too smooth, the distribution range of the reconstruction result is large, far bey

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[0044] The present invention will be described below in conjunction with the accompanying drawings.

[0045] Such as figure 1 As shown, the present invention is a bioluminescence tomography reconstruction method, which includes the following steps:

[0046] (1) Data collection and preprocessing;

[0047] First, a small animal is injected intraperitoneally or intravenously with the substrate luciferin, and the luciferase reacts with the substrate luciferin in the organism to produce fluorescent photons. Fix the organism and collect optical data such as multi-angle fluorescence and white light in the dark room using the BLT system. A CT system is used to scan the organism to obtain scan data and geometric correction data.

[0048] Perform format conversion and background denoising processing on the collected BLT fluorescence data, and then superimpose the obtained fluorescence data and white light data to obtain new superimposed data.

[0049] Preprocess the collected CT data. Process ...

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Abstract

The invention discloses a bioluminescence tomography reconstruction method. The method includes the steps: acquiring signal distribution conditions of a bioluminescent light source in a small animal body on a body surface by the aid of a bioluminescence tomography data acquisition platform and acquiring anatomical structure information in the small animal body by the aid of a Micro-CT (computed tomography) system; and constructing a system equation based on a three-order simplified spherical harmonic approximation model of a radiative transport equation and a finite-element mesh, and solving the system equation by a compressive sensing sparse regularization method to obtain accurate positioning information of the light source in the small animal body. By the aid of the bioluminescence tomography reconstruction method, three-dimensional light source distribution and the positioning information in the small animal body can be inversed through the two-dimensional light source distribution and the anatomical structure information of the small animal body surface.

Description

technical field [0001] The present invention relates to the technical field of optical imaging, in particular to a bioluminescent tomographic imaging method. The present invention is based on a third-order simplified spherical harmonic approximation model and a sparse regularization method, and can be applied to early diagnosis of diseases, gene therapy and drug research and development, etc. field. Background technique [0002] In recent years, due to its advantages over X-ray imaging, magnetic resonance imaging, and nuclide imaging in terms of sensitivity, safety, operability, and cost, optical imaging technology has developed rapidly and is widely used in biological research. Remarkable scientific research results have been obtained. Among them, bioluminescence tomography (Bioluminescence tomography, BLT), as a leading optical imaging technology, collects optical signals through a high-sensitivity CCD camera, combines the light transmission theory of biological tissue an...

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Application Information

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IPC IPC(8): A61B5/00A61B6/03
CPCA61B6/508
Inventor 陈雪利张岐坦梁继民杨德富曹欣陈多芳朱守平屈晓超田捷
Owner GUANGZHOU BLT INSTR & METER
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