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Oocyte in-vitro maturity nutrient solution and preparation method thereof

A technology for in vitro maturation culture and oocyte, which is applied to the field of in vitro maturation of oocyte and its preparation, and can solve the problems of inability to industrialize, unclear components of refinement, unstable clinical results, etc., and achieve stable quality and composition. Stable, stable and safe effect of commercial components

Active Publication Date: 2014-07-09
JIANGSU HAOKANG BIOLOGICAL MEDICAL SCI & TECH CO LTD
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of oocyte maturation medium and its preparation method in vitro with stable composition and stable quality, which can be produced industrially, so as to solve the problems in the prior art due to the unclear composition of various additives. , resulting in unstable clinical results and inability to industrialize

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  • Oocyte in-vitro maturity nutrient solution and preparation method thereof
  • Oocyte in-vitro maturity nutrient solution and preparation method thereof
  • Oocyte in-vitro maturity nutrient solution and preparation method thereof

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Embodiment Construction

[0013] 1. Preparation of oocyte maturation medium in vitro

[0014] The preparation method of the oocyte in vitro maturation culture solution of the present invention:

[0015] Add 0.075-0.10IU / ml FSH and 0.5-0.8IU / ml HCG to the basic cell culture medium TCM199, filter it with a 0.22μm filter, store it at 4°C, and use it within 3 weeks. Before use, add 0.1-0.2μg / ml 17β-estradiol, 10%-20% human commercial substitute serum or human albumin, and mix thoroughly. Before culturing oocytes, the culture solution is equilibrated in a 37°C incubator with 5% carbon dioxide concentration for more than 12 hours.

[0016] Addition and configuration are required to be carried out in a GMP workshop environment, which is sterile, non-toxic and dust-free.

[0017] 2. Acute toxicity and long-term toxicity test of oocyte maturation medium in vitro

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Abstract

The invention belongs to the technical field of assisted reproduction, and particularly relates to a oocyte in-vitro maturity nutrient solution and a preparation method thereof. The oocyte in vitro manturation culture medium is based on commercialized tissue nutrient solution TCM199, and accurately added commercialized constituents comprise 0.075-0.10 IU / ml FSH and 0.5-0.8 IU / ml HCG, 0.1-0.2 microgram / ml 17 beta-estradiol, and human commercialized replaced serum or human albumin with the volume percent being of 10-20 percent. The oocyte in-vitro maturity nutrient solution is stable in constituents and quality, can be industrially produced. By adding commercialized human replaced serum and serum albumin, improving the concentration of the estradiol, FSH and HCG in the culture medium and simulating in-vivo mature environment in human oocyte, a good clinical effect is obtained, the in vitro maturity rate of oocyte reaches 72.08 percent, and the clinical pregnancy rate reaches 23.71 percent.

Description

Technical field [0001] The invention belongs to the technical field of assisted reproduction, and specifically relates to an oocyte maturation culture solution in vitro and a preparation method thereof. Background technique [0002] Most of the existing culture media used for culturing female germ cells to mature rapidly in vitro are added with fetal bovine serum or human mature follicular fluid, which is likely to cause the spread of heterogeneous pathogens and the spread of unexplained diseases between the same species, and it is easy to cause neonatal disease. Defects are not conducive to prenatal and postnatal care. In addition, the detailed components of various additives are not clear, leading to unstable clinical results and inability to industrialize. Summary of the invention [0003] The purpose of the present invention is to provide an oocyte in vitro maturation culture solution with stable ingredients and stable quality, which can be industrially produced, and a prepar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075
Inventor 钱云陈娟
Owner JIANGSU HAOKANG BIOLOGICAL MEDICAL SCI & TECH CO LTD
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