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Process for producing ethanol and ethylene via fermentation

A self-produced ethanol Clostridium and ethanol technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of increasing factory costs and paying emission taxes

Inactive Publication Date: 2013-09-04
LANZATECH NEW ZEALAND LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, emissions taxes may need to be paid, increasing the cost of the plant

Method used

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  • Process for producing ethanol and ethylene via fermentation
  • Process for producing ethanol and ethylene via fermentation
  • Process for producing ethanol and ethylene via fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Materials and methods:

[0110]

[0111] Preparation of Cr(II) solution: A 1 L three-necked flask was fitted with gas-tight inlet and outlet so that it could be operated under inert gas and the desired product was subsequently transferred to a suitable storage flask. Add CrCl to the flask 3 ·6H 2 O (40 g, 0.15 mol), zinc particles [20 mesh] (18.3 g, 0.28 mol), mercury (13.55 g, 1 mL, 0.0676 mol) and 500 mL of distilled water. use N 2 After rinsing for one hour, the mixture was warmed to about 80°C to initiate the reaction. at constant N 2 Stirred under flow for 2 hours, then cooled the mixture to room temperature and continued to stir for 48 hours, by which time the reaction mixture had turned into a dark blue solution. Transfer the solution to N 2 Rinse the serum bottle and store in the refrigerator until use.

[0112] Bacteria: Two types of Clostridium autoethanogenum were used in the following examples.

[0113] Both DSM19630 and DSM23693 were deposited at...

Embodiment 2

[0126] Materials and methods:

[0127] Bacterial strains and growth conditions: Clostridium autoethanogenum DSM10061 and Clostridium ljungdahlii DSM13582 were obtained from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), Clostridium rasseri ATCC-BAA622 TM Obtained from ATCC (American Type Culture Collection). All organisms were grown anaerobically in modified PETC medium (ATCC medium 1754) at 30 °C (C. lasseri) or at 37 °C (C. autoethanogenum and C. ljungdahlii), respectively.

[0128] The improved PETC medium (per L) includes 1g NH Cl, 0.4g KCl, 0.2g MgSO 4 ·7H 2 O, 0.8g NaCl, 0.1g KH 2 PO 4 , 20mg CaCl 2 2H 2 O, 10ml trace element solution (see below), 10ml Wolfe's vitamin solution (see below), 2g NaHCO 3 and 1 mg resazurin. After adjusting the pH to 5.6, the medium was anaerobically boiled, dispensed, and autoclaved at 121° C. for 15 minutes. Steel mill exhaust gas (composition: 44% CO, 32% N) collected from the New Zealand steel site in Glenbrook...

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Abstract

A process for converting a substrate such as carbon monoxide to useful chemicals has been developed. The process involves providing a substrate comprising CO to a bioreactor which contains a culture of one or more micro-organisms and anaerobically fermenting the substrate to produce ethanol. The ethanol is next converted to one or more chemical products via the compound ethylene. The source of the CO can be an industrial process such as the ferrous metal products manufacturing. The microorganism can be Clostridium autoethanogenum, Clostridium ljundahlii or Clostridium ragsdalei.

Description

technical field [0001] The present invention relates to the production of one or more chemical products using a procedure comprising microbial fermentation, particularly of a substrate comprising CO. Background technique [0002] Ethylene is a high-value gaseous compound widely used in industry. For example, ethylene is used as an anesthetic or as a fruit ripening agent, as well as in the production of a variety of other chemical products. For example, ethylene can be used to produce polyethylene, ethylene oxide, ethylene dichloride, ethylene dibromide, ethyl chloride, and ethylbenzene, which in turn can be used to produce other useful downstream products. [0003] Carbon monoxide (CO) is a major by-product of incomplete combustion of organic materials such as coal or oil and oil-derived products. Although complete combustion of carbon-containing precursors produces CO 2 and water as the only end product, but some industrial processes require high 2 It is more conducive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12R1/145
CPCC07C1/24C12P7/065C12P7/08C12N1/205C12R2001/145Y02E50/10C07C11/04
Inventor S·D·辛普森C·D·米哈尔策
Owner LANZATECH NEW ZEALAND LTD
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