Method and kit for detecting lithium ions
A lithium ion and kit technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as poor repeatability, inability to timely and accurate detection results, and increased detection costs.
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Embodiment 1
[0116] Embodiment 1 makes standard curve
[0117] 1.1 Preparation of reaction reagents
[0118] 1.1.1 Prepare test reagent (1) (or called R1 reagent) according to Table 1:
[0119] Table 1
[0120]
[0121] 1.1.2 Prepare test reagent (2) (or called R2 reagent) according to Table 2:
[0122] Table 2
[0123]
[0124]
[0125] 1.2 Make standard curve
[0126] Take 200 μl of R1 reagent and 100 μl of R2 reagent prepared above, and mix them with serum samples with the following lithium ion concentrations: 0.00 mmol / L (deionized water), 1.55 mmol / L, 5 μl each. Two-point calibration is adopted, and the calibration method is linear calibration.
[0127] 1.2.1 Detection method
[0128] According to the parameters in the detection method, add 5 μl serum sample to the cuvette, then add 200 μl R1 reagent, incubate for 5 minutes, then add 100 μl R2 reagent. After three minutes, continuous detection with a wavelength of 546 nm was started.
[0129] 1.2.2 Test results
[01...
Embodiment 2
[0139] Dilute the 3.00mmol / L serum sample with normal saline at the ratio of 1 / 15, 1 / 5, 2 / 5, 3 / 5, 4 / 5, 5 / 5 to obtain diluted samples 4.1, 4.2, 4.3, 4.4, 4.5 (Theoretical concentrations are detailed in Table 5).
[0140] According to the calibration result of embodiment 1 (promptly obtained according to embodiment 1 such as image 3 The standard curve shown), to obtain the corresponding test concentration of each sample.
[0141] table 5
[0142] sample
theoretical concentration
test concentration
estimated concentration
deviation
4.1
0.20
0.21
0.195
-7.14%
4.2
0.60
0.59
0.605
2.54%
4.3
1.20
1.23
1.22
-0.81%
4.4
1.80
1.82
1.835
0.82%
4.5
2.40
2.44
2.45
0.41%
4
3.00
3.08
3.065
-0.49%
[0143] According to the theoretical concentration and the test concentration, the figure 1 shown in the linear regression plo...
Embodiment 3
[0147] According to the calibration result of embodiment 1 (i.e. the calibration curve obtained according to embodiment 1, such as image 3 shown), tested serum or blood samples from 40 patients whose blood lithium levels were being monitored.
[0148] Simultaneously, adopt ISE method, promptly measure the said 40 examples of samples on the American Easylyte electrolyte analyzer (its supporting calibrating liquid, washing liquid, purchased from American MEDICA company).
[0149] Test results such as figure 2 As shown, the results show that: the detection method of the present invention has a good correlation with the ISE method, the slope is 1.0098, and the R2 is 0.9975. (abscissa and ordinate unit: mmol / L)
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