Method for identifying protoplast monokaryon mating types of lepista sordida and special primer pair SR-4*2 thereof

A technique for protoplasts and protoplasts of Pleurotus edodes, applied in the field of identifying the mating type of protoplasts of Pleurotus edodes, can solve the problems of narrow temperature range, low yield, poor insect resistance, etc., and achieve high accuracy and reliability Effect
CN103290117BInactive Publication Date: 2014-11-05BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Publication Date
2014-11-05
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for identifying the protoplast monokaryon mating types of lepista sordida and a special primer pair SR-4*2 thereof. The method comprises the following steps of: with genome DNAs (deoxyribonucleic acids) of the protoplast monokaryons of two plants of lepista sordida to be identified as templates, performing PCR (Polymerase Chain Reaction) amplification by using a PCR primer pair composed of two single chain DNAs as shown in SEQ ID No.1 and SEQ ID No.2, and detecting the sizes of the obtained PCR products; if the PCR products of the protoplast monokaryons of the two plants of lepista sordida to be identified both contain or do not obtain 500 bp-800 bp of DNA segments, indicating that the mating types of the protoplast monokaryons of the two plants of lepista sordida are the same; and if one plant contains 500 bp-800 bp of DNA segments, while the other plant contains no 500 bp-800 bp of DNA segments, indicating that the mating types of the protoplast monokaryons of the two plants of lepista sordida are different.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for identifying the monokaryon mating type of the protoplast and the special primer pair SR-4×2 of the mushroom. Background technique

[0002] The nutrient incompatibility system is a genetic system for identifying aliens. In most fungi, this system can cause genetically distinct individuals to produce characteristic somatic incompatibility in the junction zone (Qi Yuancheng et al., Chinese cultivated pellagra Comparison of somatic cell incompatibility test and RAPD analysis results of Pleurotus species. Acta Mycophyta Sinica, 2010, 29 (3)) reaction, the intensity of somatic cell incompatibility reaction varies from individual to individual. In fungi, nutritional incompatibility is also known as somatic incompatibility or heterokaryotic incompatibility. fusion to maintain individual genetic stability. The mating type is the type of combination determined according to whether the individuals of the mating factors can ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More