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Human liver cancer cell line HLCZ02 and application thereof

A human hepatoma cell line, hepatocyte technology, applied in the field of microbial animal cells

Active Publication Date: 2013-09-18
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Transgenic mice have not been widely used in HCV immunology, and immune tolerance to viral proteins has become an issue for research using mouse animal models

Method used

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  • Human liver cancer cell line HLCZ02 and application thereof
  • Human liver cancer cell line HLCZ02 and application thereof
  • Human liver cancer cell line HLCZ02 and application thereof

Examples

Experimental program
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Embodiment

[0042] In this example, a human liver cancer cell line HLCZ02 of the present invention was obtained. The human liver cancer cell line HLCZ02 has been preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: C201310. The address of the depository unit is located at Wuhan University, China. The date of deposit is January 17, 2013, and the human liver cancer cell line HLCZ02 is named as the human liver cancer cell line HLCZ02.

[0043] Establishment and culture method of HLCZ02 cell line:

[0044] 1. Take a fresh surgical specimen from a male liver cancer patient, wash it once with PBS, and cut the tissue block into 1mm with a scalpel 3 Spread the small piece of collagen in a petri dish (100mm) that has been treated with collagen, add 8ml of fresh medium, and add epidermal growth factor at the same time, the final concentration is 10ng / ml;

[0045] 2. Change the fresh medium after 2 days of cultivation, and then change the fresh medium ...

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Abstract

The invention discloses a human liver cancer cell line HLCZ02 which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: C 201310. The human liver cancer cell line HLCZ02 disclosed by the invention can be applied to cell models or animal models, can also be used for preparing, selecting or evaluating anti-hepatitis virus medicines and anti-tumor medicines, and can also be used for preparing man-made livers.

Description

technical field [0001] The invention relates to the field of microbial animal cells, in particular to a human liver cancer cell line and its application. Background technique [0002] The liver has a variety of important physiological functions, mainly including: synthesis and secretion of a large number of serum proteins, such as albumin and lipoprotein; synthesis and transport of lipids bound to proteins; detoxification function; synthesis and secretion of bile; synthesis of sugar to regulate blood sugar; Decompose amino acids to synthesize urea; activate vitamins; synthesize and decompose glycogen; synthesize glutathione and metallothionein, etc. [0003] With the advancement of biotechnology, many useful biological products can be produced by gene recombination and cell fusion, but in comparison, animal cell culture technology is more important than before. Considering that the liver has more physiological functions than other organs, including its significant synthesis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027C12R1/91
Inventor 朱海珍左朝晖王小红杨大荣刘念礼
Owner HUNAN UNIV